A thermodynamic comparison of mesophilic and thermophilic ribonucleases H

J Hollien, Susan Marqusee


The mechanisms by which thermophilic proteins attain their increased thermostability remain unclear, as usually the sequence and structure of these proteins are very similar to those of their mesophilic homologues. To gain insight into the basis of thermostability, we have determined protein stability curves describing the temperature dependence of the free energy of unfolding for two ribonucleases H, one from the mesophile Escherichia coli and one from the thermophile Thermus thermophilus. The circular dichroism signal was monitored as a function of temperature and guanidinium chloride concentration, and the resulting free energies of unfolding were fit to the Gibbs-Helmholtz equation to obtain a set of thermodynamic parameters for these proteins. Although the maximal stabilities for these proteins occur at similar temperatures, the heat capacity of unfolding for T. thermophilus RNase H is lower, resulting in a smaller temperature dependence of the free energy of unfolding and therefore a higher thermal melting temperature. In addition, the stabilities of these proteins are similar at the optimal growth temperatures for their respective organisms, suggesting that a balance of thermodynamic stability and flexibility is importan...Continue Reading


Sep 16, 2011·Journal of Biomolecular NMR·Michelle L Gill, Arthur G Palmer
Dec 19, 2002·Biophysical Chemistry·S W EnglanderJ Rumbley
Oct 20, 2005·Journal of Bioscience and Bioengineering·S Fujiwara
Oct 20, 2005·Journal of Bioscience and Bioengineering·Kentaro ShirakiMasahiro Takagi
Apr 15, 2008·Quarterly Reviews of Biophysics·S W EnglanderMallela M G Krishna
Dec 11, 2002·European Journal of Biochemistry·Graeme S BellGarry L Taylor
Nov 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Daniel G IsomBertrand García-Moreno
Jan 9, 2007·Journal of Biomolecular Structure & Dynamics·Ling Tang, Haiyan Liu
Apr 19, 2011·Molecular Biology and Evolution·Mathieu Groussin, Manolo Gouy
May 23, 2001·Protein Science : a Publication of the Protein Society·D C Rees, A D Robertson
Jan 16, 2002·Protein Science : a Publication of the Protein Society·Srebrenka RobicSusan Marqusee
Jul 27, 2001·Protein Science : a Publication of the Protein Society·C MoczygembaP Wittung-Stafshede
Dec 6, 2000·Protein Science : a Publication of the Protein Society·L Mayne, S W Englander
Mar 10, 2001·Microbiology and Molecular Biology Reviews : MMBR·C Vieille, G J Zeikus
Jul 10, 2010·BMC Evolutionary Biology·Jun OkadaShigenori Kanaya
Mar 31, 2007·BMC Structural Biology·Richard B Greaves, Jim Warwicker
Mar 8, 2012·PLoS Computational Biology·Deeptak VermaDennis R Livesay
Apr 29, 2009·International Journal of Molecular Sciences·Atsushi Mukaiyama, Kazufumi Takano
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·J Hollien, Susan Marqusee
Sep 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Srebrenka RobicSusan Marqusee
Jan 3, 2001·Proceedings of the National Academy of Sciences of the United States of America·J RumbleyS W Englander
Apr 26, 2014·The Journal of Physical Chemistry. B·Abhishek Narayan, Athi N Naganathan
Oct 11, 2014·Protein Engineering, Design & Selection : PEDS·Sabino PachecoFrédéric Pecorari
Jan 1, 2015·Journal of Molecular Biology·Kate A StaffordArthur G Palmer
Jul 3, 2013·Protein Science : a Publication of the Protein Society·Alexander Tischer, Matthew Auton
Jul 6, 2010·Biochimica Et Biophysica Acta·Md Zahid KamalNalam Madhusudhana Rao
Nov 10, 2009·Computational Biology and Chemistry·Tienhsiung KuNaiwan Hsiao
May 13, 2008·Journal of Molecular Biology·Marcus WallgrenMagnus Wolf-Watz
Aug 25, 2004·Proteins·Brian N DominyCharles L Brooks
May 28, 2009·Protein Science : a Publication of the Protein Society·Shoeb Ahmad, Nalam Madhusudhana Rao
Jul 4, 2006·Protein Science : a Publication of the Protein Society·Abbas Razvi, J Martin Scholtz
Oct 13, 2007·Proteins·Atsushi MukaiyamaShigenori Kanaya
Nov 8, 2006·Protein Science : a Publication of the Protein Society·Joel A Butterwick, Arthur G Palmer
Feb 17, 2005·Journal of Molecular Biology·Sudharsan SridharanJames C Sacchettini
Sep 4, 2007·Archives of Biochemistry and Biophysics·Eugenia PechkovaClaudio Nicolini
May 29, 2004·Journal of Molecular Biology·Joel A ButterwickArthur G Palmer
Jan 8, 2000·Biochimica Et Biophysica Acta·L E EiseleM R Edwards
May 23, 2006·Molecular Cell·Rafael CouñagoYousif Shamoo
Mar 21, 2008·Journal of Molecular Biology·Hongju DongShigenori Kanaya
Oct 7, 2010·Biophysical Journal·James M MottonenDennis R Livesay
Apr 29, 2016·Journal of Chemical Theory and Computation·Tim ZeiskeArthur G Palmer
Nov 12, 2014·PLoS Biology·Kathryn M HartSusan Marqusee
Oct 5, 2005·PLoS Computational Biology·Igor N BerezovskyEugene I Shakhnovich
Nov 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Shion A LimSusan Marqusee
Jun 28, 2001·Preparative Biochemistry & Biotechnology·M M Gromiha
Dec 6, 2001·Journal of Molecular Biology·E R Goedken, Susan Marqusee
Feb 23, 2018·Frontiers in Molecular Biosciences·George P LisiJ Patrick Loria

Related Concepts

Circular Dichroism, Vibrational
Enzyme Stability
Alkalescens-Dispar Group
Protein Denaturation
Ribonuclease H, Calf Thymus
Thermus thermophilus (bacterium)
Homologous Sequences, Amino Acid
Guanidine Sulfite (1: 1)

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

© 2021 Meta ULC. All rights reserved