PMID: 9540837Apr 16, 1998Paper

A thermophilic nitrate reductase is responsible for the strain specific anaerobic growth of Thermus thermophilus HB8

Biochimica Et Biophysica Acta
S Ramírez-ArcosJ Berenguer

Abstract

T. thermophilus HB8 contains a nitrate reductase gene cluster which is absent from closely related strains. This cluster encodes 4 ORFs (a-d) similar in organization and protein sequence to those encoded by respiratory nitrate reductase operons (narGHJI) of Escherichia coli, Bacillus subtilis, Pseudomonas fluorescens, and Thiosphaera pantothropha. The highest similarity is shown between the proteins encoded by the ORFa, ORFb and ORFd, and the structural components of the mesophilic nitrate reductases NarG (alpha), NarH (beta), and NarI (gamma) proteins, whilst ORFc encodes a protein which showed lower similarity to NarJ, a protein of unknown function encoded between narH and narI genes in all the nar cluster so far sequenced. This T. thermophilus HB8 narGHJI cluster is strongly induced by the combined effect of nitrate and low oxygen concentration, giving rise to the synthesis of an enzyme whose optimal temperature and pH was determined to be 80 degrees C, and pH 10, respectively. We also demonstrate that insertional inactivation of the narG and narH genes of this cluster results in strictly aerobic mutants, showing its sole responsibility in the strain specific ability of T. thermophilus HB8 to grow anaerobically.

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Citations

Feb 28, 2009·Extremophiles : Life Under Extreme Conditions·Kyle C CostaBrian P Hedlund
Jul 9, 1999·Biochimica Et Biophysica Acta·L Philippot, O Højberg
Jul 3, 2007·Applied and Environmental Microbiology·Emilio Blas-GalindoJosé Berenguer
Dec 21, 2010·Applied and Environmental Microbiology·Laura AlvarezJosé Berenguer
Aug 5, 2003·Journal of Bacteriology·Pablo CastánJosé Berenguer
Dec 3, 2003·Journal of Bacteriology·Charles D Sohaskey, Lawrence G Wayne
Nov 21, 2007·Biochemistry. Biokhimii︠a︡·E V Morozkina, R A Zvyagilskaya
Apr 25, 2012·Extremophiles : Life Under Extreme Conditions·Federico AcostaJosé Berenguer
Jan 4, 2012·Extremophiles : Life Under Extreme Conditions·Federico AcostaJosé Berenguer
Oct 22, 2013·Applied and Environmental Microbiology·Laura AlvarezJosé Berenguer
Mar 28, 2009·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marcos AlmendrosJosé Berenguer Carlos
Feb 18, 2015·Chemistry : a European Journal·Constantinos KoutsoupakisConstantinos Varotsis
Mar 6, 2012·Biochimica Et Biophysica Acta·Mohamed Radzi Noor, Tewfik Soulimane
Feb 5, 2013·Biochimica Et Biophysica Acta·Stéphane GrimaldiAxel Magalon
Apr 6, 2004·Nature Biotechnology·Anke HenneHans-Joachim Fritz
Apr 22, 2006·The EMBO Journal·Oleg LaptenkoSergei Borukhov
Jan 21, 2014·Journal of Bacteriology·Laura AlvarezJosé Berenguer
Jun 8, 2014·Applied and Environmental Microbiology·C BricioJ Berenguer
Sep 7, 2018·Environmental Microbiology·Zahra ChahlafiJosé Berenguer
Dec 4, 2001·FEMS Microbiology Letters·T M Gihring, J F Banfield
Nov 6, 2004·Microbiology·Purificación CabelloConrado Moreno-Vivián
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Feb 28, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Laura AlvarezJosé Berenguer
Mar 2, 1999·Applied and Environmental Microbiology·T L KieftM S Gray

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