A Thermostable Protein Matrix for Spectroscopic Analysis of Organic Semiconductors.

Journal of the American Chemical Society
George A SutherlandC Neil Hunter

Abstract

Advances in protein design and engineering have yielded peptide assemblies with enhanced and non-native functionalities. Here, various molecular organic semiconductors (OSCs), with known excitonic up- and down-conversion properties, are attached to a de novo-designed protein, conferring entirely novel functions on the peptide scaffolds. The protein-OSC complexes form similarly sized, stable, water-soluble nanoparticles that are robust to cryogenic freezing and processing into the solid-state. The peptide matrix enables the formation of protein-OSC-trehalose glasses that fix the proteins in their folded states under oxygen-limited conditions. The encapsulation dramatically enhances the stability of protein-OSC complexes to photodamage, increasing the lifetime of the chromophores from several hours to more than 10 weeks under constant illumination. Comparison of the photophysical properties of astaxanthin aggregates in mixed-solvent systems and proteins shows that the peptide environment does not alter the underlying electronic processes of the incorporated materials, exemplified here by singlet exciton fission followed by separation into weakly bound, localized triplets. This adaptable protein-based approach lays the foundation ...Continue Reading

References

Feb 3, 1989·Science·W F DeGradoJ D Lear
Mar 31, 1994·Nature·D E RobertsonP L Dutton
Nov 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·G M Edelman, J A Gally
Feb 19, 2003·Journal of Molecular Biology·Steve S HuangMitchell Lewis
Feb 24, 2004·Molecular and Cellular Biochemistry·José G Sampedro, Salvador Uribe
Jul 13, 2006·The Journal of Physical Chemistry. a·Helena Hörvin BillstenTomás Polívka
Nov 30, 2007·Angewandte Chemie·John E Anthony
Jan 30, 2009·Protein Science : a Publication of the Protein Society·Nishant Kumar Jain, Ipsita Roy
Mar 20, 2009·Nature·Ronald L KoderP Leslie Dutton
Apr 29, 2009·Journal of Computational Chemistry·Garrett M MorrisArthur J Olson
Sep 23, 2010·Journal of the American Chemical Society·Chen Wang, Michael J Tauber
Feb 17, 2011·Faraday Discussions·P Leslie Dutton, Christopher C Moser
Jan 10, 2013·Annual Review of Physical Chemistry·Millicent B Smith, Josef Michl
Oct 15, 2013·Nature Chemical Biology·Tammer A FaridP Leslie Dutton
Jan 16, 2014·Annual Review of Physical Chemistry·Frank C Spano, Carlos Silva
Feb 6, 2014·Journal of the American Chemical Society·Lee A SolomonP Leslie Dutton
Apr 1, 2015·Journal of the American Chemical Society·Andrew J MusserJenny Clark
Jun 24, 2015·Journal of the American Chemical Society·Samuel N SandersLuis M Campos
Aug 12, 2015·The Journal of Physical Chemistry Letters·Geoffrey B Piland, Christopher J Bardeen
Aug 13, 2015·The Journal of Physical Chemistry Letters·Murad J Y TayebjeeTimothy W Schmidt
Dec 24, 2015·Journal of the American Chemical Society·Nadezhda V KorovinaMark E Thompson
Jun 10, 2016·ACS Central Science·Eric G FuemmelerNandini Ananth
Sep 3, 2016·Methods in Enzymology·C C MoserP L Dutton
Sep 16, 2016·Nature·Po-Ssu HuangDavid Baker
Oct 1, 2016·Nano Letters·Anna TsargorodskaGraham J Leggett
Dec 14, 2016·Journal of the American Chemical Society·Chris BialasP J Hore
Feb 10, 2017·Journal of the Royal Society, Interface·Joshua A ManciniChristopher C Moser
Mar 7, 2017·Chemical Science·Goutham KodaliChristopher C Moser
Aug 27, 2017·Nature Communications·Daniel W WatkinsJ L Ross Anderson
Sep 5, 2017·Physical Chemistry Chemical Physics : PCCP·Eric R KennehanJohn B Asbury
Apr 6, 2018·Journal of the Royal Society, Interface·Joshua A ManciniChristopher C Moser
Apr 6, 2019·Proceedings of the National Academy of Sciences of the United States of America·Michelle ChenMichael R Wasielewski
Apr 25, 2019·Chemical Science·Andrew B PunDaniel N Congreve
Jun 9, 2019·Annual Review of Physical Chemistry·Andrew J Musser, Jenny Clark
Aug 3, 2019·The Journal of Chemical Physics·Andrew J MusserJenny Clark
Mar 4, 2020·Nature Chemistry·Nadezhda V KorovinaJustin C Johnson

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