PMID: 9440542Jan 24, 1998Paper

A thiol antioxidant regulates IgE isotype switching by inhibiting activation of nuclear factor-kappaB

The Journal of Allergy and Clinical Immunology
Y YanagiharaK Ikizawa

Abstract

The binding site for nuclear factor-kappaB (NF-kappaB) is present at the promoter region of the germline Cepsilon gene, but there is little information on whether this factor is involved in regulating IgE synthesis by human B cells. Accordingly, we studied the role of NF-kappaB in germline Cepsilon transcription by using two human Burkitt's lymphoma B cell lines, DND39 and DG75. In both cell lines, n-acetyl-L-cysteine (NAC), a potent thiol antioxidant, inhibited the triggering of the nuclear expression of NF-kappaB by IL-4 and by anti-CD40 monoclonal antibody. Although IL-4 activated signal transducers and activators of transcription (STAT) 6 in addition to NF-kappaB, NAC treatment or the transfection of decoy oligodeoxynucleotides for NF-kappaB or STAT6 only partly blocked IL-4-induced germline Cepsilon transcription. However, these two decoy oligodeoxynucleotides together almost completely abrogated IL-4-induced germline Cepsilon transcription. Of note, CD40-mediated enhancement of IL-4-driven germline Cepsilon transcription was markedly decreased by NAC or by a decoy oligodeoxynucleotide for NF-kappaB. The effect of NAC was also examined on deletional switch recombination underlying the isotype switch to IgE. NAC inhibited t...Continue Reading

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Citations

Oct 5, 2007·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yasuhiro Matsumura
Dec 31, 2010·Anatomy & Cell Biology·Ami WooYoung-Il Hwang
Nov 3, 2009·Biochemical and Biophysical Research Communications·Sophie CurboMathias Lundberg

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