A Thioredoxin Homologous Protein of Plasmodium falciparum Participates in Erythrocyte Invasion

Infection and Immunity
Wei WangQijun Chen

Abstract

Invasion of erythrocytes by merozoites is required in the life cycle of malarial parasites. Proteins derived from the invasive merozoites are essential ligands for erythrocyte recognition and penetration. In this study, we report a novel protein that possesses a Trx domain-like structure of the thioredoxin family and is expressed on the surface of merozoites of the malaria parasite Plasmodium falciparum This protein, namely, PfTrx-mero protein, displayed a mutated sequence character at the Trx domain, but with a specific binding activity to human erythrocytes. Specific antibodies to the protein inhibited merozoite invasion into human erythrocytes. Immunization with a homologous protein of Plasmodium berghei strain ANKA also showed significant protection against lethal infection in mice. These results suggested that the novel PfTrx-like-mero protein expressed on the surface of merozoites is an important ligand participating in erythrocyte invasion and a potential vaccine candidate.

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Citations

Jan 19, 2021·Parasitology Research·Savitri TiwariNeelima Mishra

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Methods Mentioned

BETA
PCR
affinity purification
enzyme-linked immunosorbent assay

Software Mentioned

TMHMM
Basic Local Alignment Search Tool ( BLAST )
SignalP
DNAman

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Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.