PMID: 7061384Mar 1, 1982

A third DNA polymerase from Spiroplasma citri and two other spiroplasmas

Journal of Bacteriology
A CharronJ M Bove


Recently, two DNA polymerases (ScA and ScB) were isolated and characterized from Spiroplasma citri. We now have found a third DNA polymerase (ScC) not only in S. citri but also in the serologically related honeybee spiroplasma BC3 and the unrelated flower spiroplasma BNR1. Enzyme ScC is N-ethylmaleimide (NEM) sensitive. The three DNA polymerases from the honeybee spiroplasma seem to be similar to the respective enzymes of S. citri. However, whereas the NEM-resistant enzyme ScA from S. citri and that from the BC3 honeybee spiroplasma are retained on DEAE-cellulose and require 0.09 M KCl for elution, the NEM-resistant enzyme A from the flower spiroplasma BNR1 is not retained.


Jan 1, 1996·Annual Review of Microbiology·K Dybvig, L L Voelker

Related Concepts

Chromatography, DEAE-Cellulose
DNA Polymerase N3

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