A third secreted protein that is encoded by the enteropathogenic Escherichia coli pathogenicity island is required for transduction of signals and for attaching and effacing activities in host cells.

Infection and Immunity
L C LaiM S Donnenberg

Abstract

Enteropathogenic Escherichia coli strains are able to signal host cells, cause dramatic cytoskeletal rearrangements, and adhere intimately to the cell surface in a process known as the attaching and effacing effect. A pathogenicity island of 35 kb known as the locus of enterocyte effacement (LEE) is necessary and sufficient for this effect. The LEE encodes an outer membrane adhesin called intimin, a type III secretion apparatus, and the EspA and EspB secreted proteins. The DNA sequence of the region between espA and espB revealed a new gene, espD. The product of espD was demonstrated by using a T7 expression system. We constructed a nonpolar mutation in espD and found that the mutant is incapable of the signal transduction events that lead to activation of the putative intimin receptor in host cells and that the mutant fails to induce the attaching and effacing effect. These phenotypes were restored to the mutant by complementation with a plasmid containing the cloned espD locus. We demonstrated by immunoblotting and microsequencing that the EspD protein is secreted via the type III apparatus. Thus, we describe a novel locus encoding a secreted protein that is required for attaching and effacing activity.

References

Sep 1, 1990·Infection and Immunity·T MichielsG Cornelis
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Jan 1, 1995·Methods in Enzymology·M S Donnenberg, J P Nataro
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·B Kenny, B B Finlay
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·K G JarvisJ B Kaper
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·T K McDanielJ B Kaper
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·M P SoryG R Cornelis
Nov 29, 1996·Cell·E A Groisman, H Ochman
Oct 1, 1996·Emerging Infectious Diseases·J J Mecsas, E J Strauss
Dec 1, 1996·Molecular Microbiology·T L YahrD W Frank

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Citations

Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·V SperandioJ B Kaper
Apr 12, 2001·Cellular Microbiology·R K ShawS Knutton
Mar 10, 2001·The Journal of Clinical Investigation·B P McNamaraG Hecht
Apr 16, 2002·Cellular Microbiology·Toshio KodamaTakeshi Honda
Jul 15, 2003·Clinical Microbiology Reviews·S C ClarkeP H Williams
Mar 12, 2004·Annual Review of Phytopathology·S Y He
Jan 18, 2005·FEMS Microbiology Reviews·Huiwen Deborah Chen, Gad Frankel
Oct 7, 2005·Microbiology·Hao-Jie Chiu, Wan-Jr Syu
May 19, 2006·Journal of Bacteriology·Cristiano G MoreiraVanessa Sperandio
Nov 17, 1998·The Journal of Experimental Medicine·A AbeB Brett Finlay
Apr 20, 2001·Critical Reviews in Biotechnology·S ParkR A Durst
Jun 26, 2002·Journal of Pediatric Gastroenterology and Nutrition·Alan D Phillips
Dec 28, 2004·Nature Structural & Molecular Biology·Calvin K YipNatalie C J Strynadka
Jan 24, 2006·Infection and Immunity·Wensheng Luo, Michael S Donnenberg
Sep 2, 2015·The Journal of Biological Chemistry·Abhishek ChatterjeeArmando Jardim
Jul 6, 2000·Symposium Series·H Chart
Apr 5, 2005·Journal of Bacteriology·Valérie F CrepinGad Frankel
Dec 15, 2000·Molecular Microbiology·V SperandioJ B Kaper
Oct 14, 2000·Annual Review of Cell and Developmental Biology·D L GoosneyB B Finlay

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