A three-dimensional hierarchical scaffold fabricated by a combined rapid prototyping technique and electrospinning process to expand hematopoietic stem/progenitor cells

Biotechnology Letters
Yun Gyeong KangJung-Woog Shin

Abstract

To investigate the expansion of hematopoietic stem/progenitor cells (HSPCs) from umbilical cord blood using extracellular matrix (ECM) protein-coated three-dimensional hierarchical scaffolds. The expansion of HSPCs was evaluated through total nucleated cell (TNC) expansion, immuno-phenotypic analysis, and clonogenic ability. After 7 days of culture, three-dimensional cultures with fibronectin-coated scaffolds achieved the highest fold increase in TNCs (164 ± 6.9 fold) and the highest CD45(+)CD34(+) (35 %) and CD34(+)CD38(-) (32 %) ratios. Three-dimensional hierarchical scaffolds were coated with ECM protein to simulate a biomimetic environment or niche, and had a significant effect on the expansion potential of HSPCs without changing their phenotype.

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Citations

Oct 25, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Wei LiuDaping Wang
Mar 5, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Ji Eun KimJung-Woog Shin
May 28, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Michael R Nelson, Krishnendu Roy
Apr 5, 2021·Acta Biomaterialia·Chandralekha ChatterjeeCornelia Lee-Thedieck
Jul 8, 2021·Cellular and Molecular Life Sciences : CMLS·Honghu LiHe Huang

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