A three-dimensional model of a group II intron RNA and its interaction with the intron-encoded reverse transcriptase.

Molecular Cell
Lixin DaiSteven Zimmerly

Abstract

Group II introns are self-splicing ribozymes believed to be the ancestors of spliceosomal introns. Many group II introns encode reverse transcriptases that promote both RNA splicing and intron mobility to new genomic sites. Here we used a circular permutation and crosslinking method to establish 16 intramolecular distance relationships within the mobile Lactococcus lactis Ll.LtrB-DeltaORF intron. Using these new constraints together with 13 established tertiary interactions and eight published crosslinks, we modeled a complete three-dimensional structure of the intron. We also used the circular permutation strategy to map RNA-protein interaction sites through fluorescence quenching and crosslinking assays. Our model provides a comprehensive structural framework for understanding the function of group II ribozymes, their natural structural variations, and the mechanisms by which the intron-encoded protein promotes RNA splicing and intron mobility. The model also suggests an arrangement of active site elements that may be conserved in the spliceosome.

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Citations

Feb 2, 2010·Nature Chemical Biology·Michael RoitzschAnna Marie Pyle
Apr 10, 2012·Nature Structural & Molecular Biology·Russell T ChanNavtej Toor
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Reimo ZoschkeChristian Schmitz-Linneweber
Sep 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·David M TruongAlan M Lambowitz
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Jul 10, 2010·Nucleic Acids Research·Benoît CastandetAlejandro Araya
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Oct 18, 2013·RNA·Daniel LaiIrmtraud M Meyer
Nov 10, 2013·Nucleic Acids Research·Srinivas SomarowthuAnna Marie Pyle
May 8, 2010·Critical Reviews in Biochemistry and Molecular Biology·Anna Marie Pyle
May 16, 2009·Current Opinion in Structural Biology·Navtej ToorAnna Marie Pyle
Mar 21, 2009·Trends in Biochemical Sciences·François MichelEric Westhof
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