PMID: 9171080Jun 15, 1997Paper

A three-dimensional working model for a guide RNA from Trypanosoma brucei

Nucleic Acids Research
T HermannH Ulrich Göringer

Abstract

RNA editing in protozoan parasites is a mitochondrial RNA processing reaction in which exclusively uridylate residues are inserted into, and less frequently deleted from, pre-mRNAs. Molecules central to the process are so-called guide RNAs (gRNAs) which function as templates in the reaction. For a detailed molecular understanding of the mechanism of the editing process knowledge of structural features of gRNAs will be essential. Here we report on a computer-assisted molecular modelling approach to construct the first three-dimensional gRNA model for gND7-506, a ND7-specific gRNA from Trypanosoma brucei. The modelling process relied on chemical modification and enzymatic probing data and was validated by in vitro mutagenesis experiments. The model predicts a reasonably compact structure, where two stem/loop secondary structure elements are brought into close proximity by a triple A tertiary interaction, forming a core element within the centre of the molecule. The model further suggests that the surface of the gRNA is primarily made up of the sugar-phoshate backbone. On the basis of the model, footprinting experiments of gND7-506 in a complex with the gRNA binding protein gBP21 could successfully be interpreted and provide a fir...Continue Reading

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Citations

Jun 5, 2012·The Journal of Biological Chemistry·Cordula BöhmH Ulrich Göringer
Mar 17, 2001·The EMBO Journal·U F MüllerH U Göringer
Feb 21, 1998·Nucleic Acids Research·A E Souza, H U Göringer
Dec 10, 1998·Nucleic Acids Research·S Hinz, H U Göringer
Jan 16, 1999·Nucleic Acids Research·S S Leung, D J Koslowsky
Feb 10, 2000·Nucleic Acids Research·M PelletierL K Read
Jan 15, 2002·Nucleic Acids Research·Ulrich F Müller, H Ulrich Göringer
Feb 9, 2007·Nucleic Acids Research·Monika J MadejAlexander Hüttenhofer
Jan 12, 2001·Molecular and Cellular Biology·J Cruz-ReyesB Sollner-Webb
May 23, 2007·Molekuliarnaia biologiia·R Afasizhev
Sep 22, 2012·Annual Review of Microbiology·H Ulrich Göringer
Aug 1, 2015·FEBS Letters·Anna CzerwoniecJanusz M Bujnicki
Oct 1, 2011·Wiley Interdisciplinary Reviews. RNA·H Ulrich GöringerCordula Böhm
Feb 26, 2008·International Journal for Parasitology·Alena ZíkováJulius Lukes
Apr 17, 2013·Biochimica Et Biophysica Acta·Elisabeth KruseH Ulrich Göringer
Apr 16, 2010·The Journal of Biological Chemistry·Mareen SpreheMaria A Schumacher
Apr 17, 1999·The Journal of Biological Chemistry·M L Hayman, L K Read
Aug 7, 2020·Nucleic Acids Research·Cristian Del CampoH Ulrich Göringer
May 24, 2017·Nucleic Acids Research·Rachel M SimpsonLaurie K Read
Dec 14, 2018·Nucleic Acids Research·Brady TravisMaria A Schumacher
Dec 18, 2020·Nucleic Acids Research·Yanqing GaoJianhua Gan

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