A threshold level of NFATc1 activity facilitates thymocyte differentiation and opposes notch-driven leukaemia development

Nature Communications
Stefan Klein-HesslingAmiya Kumar Patra

Abstract

NFATc1 plays a critical role in double-negative thymocyte survival and differentiation. However, the signals that regulate Nfatc1 expression are incompletely characterized. Here we show a developmental stage-specific differential expression pattern of Nfatc1 driven by the distal (P1) or proximal (P2) promoters in thymocytes. Whereas, preTCR-negative thymocytes exhibit only P2 promoter-derived Nfatc1β expression, preTCR-positive thymocytes express both Nfatc1β and P1 promoter-derived Nfatc1α transcripts. Inducing NFATc1α activity from P1 promoter in preTCR-negative thymocytes, in addition to the NFATc1β from P2 promoter impairs thymocyte development resulting in severe T-cell lymphopenia. In addition, we show that NFATc1 activity suppresses the B-lineage potential of immature thymocytes, and consolidates their differentiation to T cells. Further, in the pTCR-positive DN3 cells, a threshold level of NFATc1 activity is vital in facilitating T-cell differentiation and to prevent Notch3-induced T-acute lymphoblastic leukaemia. Altogether, our results show NFATc1 activity is crucial in determining the T-cell fate of thymocytes.

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Citations

Mar 9, 2018·Oncotarget·Sabrina GiampaoloAmiya K Patra
Jun 28, 2018·Frontiers in Immunology·Johannes BaurAndris Avots
Jun 17, 2018·Cellular & Molecular Immunology·Sabrina GiampaoloAmiya K Patra
Feb 9, 2018·Frontiers in Immunology·Khalid MuhammadEdgar Serfling

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Datasets Mentioned

BETA
GSE44578
GSE55635

Methods Mentioned

BETA
nuclear translocation
RNA-seq
transgenic
ChIP-Seq
FACS
ChIP
PCR
biopsies
ChIPs
flow cytometry

Software Mentioned

BiQ Analyzer
FlowJo
CellQuest
FACSCalibur

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