Abstract
The effects of L-arginine and L-NG-nitro arginine methyl ester (L-NAME) on human blood coagulation and fibrinolysis were studied in vitro using computerized thromboelastography and native whole blood. L-Arginine (8-80 microM) prolonged the split point (SP), reaction time (R) and biKoatugulierung time (K); and diminished the angle (alpha), maximum amplitude (MA) and TEG index. L-NAME (0.5-50 microM) shortened SP, R and K and increased alpha, MA and the TEG index in a concentration-dependent manner. Neither L-arginine nor L-NAME had any effect on clot lysis. SP and R indicated the initiation of fibrin-strand formation, therefore L-arginine delayed, while L-NAME promoted the processes leading to fibrin formation. K and alpha reflect the rate of clot formation and involve fibrin build-up and platelets. Thus, L-arginine inhibited and L-NAME enhanced the rate of clot formation. MA (clot strength) involves the integrity of fibrin strands and platelet aggregation, and again L-arginine was inhibitory, while L-NAME enhanced this interaction. The TEG index indicates the coagulability of the sample; L-arginine was anticoagulant while L-NAME had procoagulant effects. These results are consistent with the inhibitory effects of NO on platelet...Continue Reading
Citations
Oct 2, 2003·Brain Research·Erin L Heinzen, Gary M Pollack
Dec 1, 2001·Thrombosis Research·T W StiefH Renz
Apr 20, 2001·British Journal of Pharmacology·J H CranshawJ A Mitchell
Jul 9, 2003·Critical Care Medicine·Jens M KlingeJörg Dötsch
May 9, 2012·Thrombosis Journal·Hans-Georg TopfJens M Klinge
Dec 23, 2006·Journal of Ethnobiology and Ethnomedicine·Kebu Balemie, Fassil Kebebew
Oct 12, 2013·PloS One·Aaron TanAlexander M Seifalian
Dec 18, 2013·Peptides·Nashwa N KabilMaha M Gamal-Eldin
May 27, 2014·Nitric Oxide : Biology and Chemistry·J W ParkA N Schechter
Mar 1, 2012·Journal of Cardiovascular Pharmacology·Murugesan Saravanakumar, Boobalan Raja
Aug 15, 2006·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Germán R Iturry-YamamotoPaulo D Picon
May 7, 2008·European Journal of Pharmacology·Zhiyu TangShiqi Peng
Feb 12, 2008·Free Radical Biology & Medicine·Katherine C WoodMark T Gladwin
Aug 26, 1998·Biochemical and Biophysical Research Communications·M V CataniG Melino
May 16, 2002·Nitric Oxide : Biology and Chemistry·M G FerriniN F Gonzalez-Cadavid
Apr 22, 2015·PloS One·Mirjana StupnisekPredrag Sikiric
Nov 10, 2013·Pharmacology, Biochemistry, and Behavior·Lee Ming-TattMohd Roslan Sulaiman
Jul 29, 2006·Journal of the Society for Gynecologic Investigation·Zeynep AlpayMichael P Diamond
Nov 28, 2002·International Journal of Impotence Research·N F Gonzalez-CadavidJ Rajfer
Mar 13, 2001·Journal of Cardiovascular Pharmacology·A PinelliE Bonacina
Apr 5, 2018·Clinical Hemorheology and Microcirculation·Ashar Usmani, Roberto F Machado
Aug 7, 2002·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Arnaldo PinelliEdgardo Bonacina