A time-resolved Fourier transformed infrared difference spectroscopy study of the sarcoplasmic reticulum Ca(2+)-ATPase: kinetics of the high-affinity calcium binding at low temperature

Biophysical Journal
A TroullierY Dupont

Abstract

We have used time-resolved Fourier transformed infrared difference spectroscopy to characterize the amplitude, frequency, and kinetics of the absorbance changes induced in the infrared (IR) spectrum of sarcoplasmic reticulum Ca(2+)-ATPase by calcium binding at the high-affinity transport sites. 1-(2-Nitro-4,5-dimethoxyphenyl)-N,N,N',N'-tetrakis [(oxycarbonyl)methyl]-1,2-ethanediamine (DM-nitrophen) was used as a caged-calcium compound to trigger the release of calcium in the IR samples. Calcium binding to Ca(2+)-ATPase induces the appearance of spectral bands in difference spectra that are all absent in the presence of the inhibitor thapsigargin. Spectral bands above 1700 cm-1 indicate that glutamic and/or aspartic acid side chains are deprotonated upon calcium binding, whereas other bands may be induced by reactions of asparagine, glutamine, and tyrosine residues. Some of the bands appearing in the 1690-1610 cm-1 region arise from modifications of peptide backbone carbonyl groups. The band at 1653 cm-1 is a candidate for a change in an alpha-helix, whereas other bands could arise from modifications of random, turn, or beta-sheet structures or from main-chain carbonyl groups playing the role of calcium ligands. Only a few resid...Continue Reading

References

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Citations

Jan 7, 2004·Journal of Photochemistry and Photobiology. B, Biology·Nan WangZong Jie Cui
Dec 22, 1999·Progress in Biophysics and Molecular Biology·J L Arrondo, F M Goñi
Jul 4, 2001·European Journal of Biochemistry·F ScheirlinckxE Goormaghtigh
Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Till RudackKlaus Gerwert
Feb 8, 2005·Biochemical and Biophysical Research Communications·Le ZhangGerard Azzar
Nov 16, 2013·The Journal of Physical Chemistry. B·Nadejda Eremina, Andreas Barth
Feb 16, 2008·Journal of Structural Biology·Tomohiro NishizawaYoshinori Fujiyoshi
Jun 30, 2005·Biophysical Journal·Bridgette A BarryDavid L Jenson
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Nov 24, 2017·Biopolymers·H RavishankarM Andersson
Mar 24, 2020·Chemical Reviews·Victor A Lorenz-Fonfria
Aug 19, 2007·Biochimica Et Biophysica Acta·Andreas Barth

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