Abstract
Undifferentiated progenitor cells of mandibular condyles of neonatal mice were kept in a tissue culture system for up to 9 days. After 2 days in culture, new chondroblasts developed within the explants, whereas the peripheries of the latter were occupied by undifferentiated cells undergoing mitosis. By 4 days in culture, many of the cartilage cells showed signs of hypertrophy, while the matrix revealed positive reactivity for type II collagen and matrix metachromasia. The process of maturation of cartilage cells appeared to have reached its final stages after 6 days in culture, at a time when the initial loci of matrix mineralization could be easily identified. Concomitantly, peripheral areas bordering the cartilaginous core, as well as portions of the cartilage, reacted positively for type I collagen and fibronectin. Light microscopy examination showed signs of new bone formation after 9 days in culture. The extracellular matrix at the upper portion of the explant, facing the medium-air interface, reacted intensely with antibodies against type I collagen and fibronectin, but not with antibodies against type II collagen. Further, the newly formed osteoid was found to have undergone mineralization, thus forming an expanded layer...Continue Reading
References
Jan 1, 1979·Journal of Immunological Methods·B Gosslau, H J Barrach
Jan 1, 1979·Cell and Tissue Research·J Thyberg, S Moskalewski
Jun 9, 1977·Nature·K von der MarkP Müller
Feb 1, 1976·Developmental Biology·H von der MarkS Gay
Oct 1, 1980·Arthritis and Rheumatism·G L Wong
Feb 1, 1981·Collagen and Related Research·H SunadaY Nagai
Aug 1, 1983·The Anatomical Record·M SilbermannK von der Mark
Jan 1, 1982·Calcified Tissue International·H C Tenenbaum, J N Heersche
Jan 1, 1983·The Journal of Cell Biology·H SudoS Kasai
Jan 1, 1981·Acta Anatomica·B K Hall
Jan 1, 1980·Acta Anatomica·P Scott-Savage, B K Hall
Citations
May 1, 1996·The Anatomical Record·K IshizekiF Suzuki
Jun 1, 1993·Calcified Tissue International·L Salo, T Kantomaa
Jan 1, 1989·Calcified Tissue International·E LivneV Erfle
Sep 1, 1996·Calcified Tissue International·D W Haas, M F Holick
Jun 30, 1994·Biochimica Et Biophysica Acta·E K BasdraA G Papavassiliou
May 29, 1998·Archives of Oral Biology·K IshizekiT Nawa
Oct 1, 1987·Collagen and Related Research·G MaorM Silbermann
Apr 30, 1999·Bone·A MolténiP J Marie
Feb 1, 2003·Anatomical Science International·Hiroyuki InoueKiyoto Ishizeki
Oct 1, 2009·Archives of Histology and Cytology·Kiyoto IshizekiHidemitsu Harada
Apr 1, 1993·Calcified Tissue International·G MaorZ Laron
Oct 1, 1987·Calcified Tissue International·M SilbermannV Erfle
Dec 20, 2011·Acta histochemica·Kiyoto Ishizeki
Apr 1, 1991·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·S B MilamD Herbert
Feb 1, 1988·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A WeissM Silbermann
Dec 3, 2016·Journal of Orofacial Orthopedics = Fortschritte Der Kieferorthopädie : Organ/official Journal Deutsche Gesellschaft Für Kieferorthopädie·Konstantinos KaramesinisChristina Piperi
Jul 26, 2005·Journal of Dental Research·G Shen, M Ali Darendeliler
Nov 1, 1990·Journal of Dental Research·F E EngelB D Boyan
Mar 19, 2020·Connective Tissue Research·Kaitlin P McCreeryCorey P Neu
Apr 18, 2015·Biology Open·Jung ParkKlaus von der Mark
May 1, 1991·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·T Kantomaa, B K Hall