A tissue culture system supporting cartilage cell differentiation, extracellular mineralization, and subsequent bone formation, using mouse condylar progenitor cells

Cell Differentiation
A WeissM Silbermann

Abstract

Undifferentiated progenitor cells of mandibular condyles of neonatal mice were kept in a tissue culture system for up to 9 days. After 2 days in culture, new chondroblasts developed within the explants, whereas the peripheries of the latter were occupied by undifferentiated cells undergoing mitosis. By 4 days in culture, many of the cartilage cells showed signs of hypertrophy, while the matrix revealed positive reactivity for type II collagen and matrix metachromasia. The process of maturation of cartilage cells appeared to have reached its final stages after 6 days in culture, at a time when the initial loci of matrix mineralization could be easily identified. Concomitantly, peripheral areas bordering the cartilaginous core, as well as portions of the cartilage, reacted positively for type I collagen and fibronectin. Light microscopy examination showed signs of new bone formation after 9 days in culture. The extracellular matrix at the upper portion of the explant, facing the medium-air interface, reacted intensely with antibodies against type I collagen and fibronectin, but not with antibodies against type II collagen. Further, the newly formed osteoid was found to have undergone mineralization, thus forming an expanded layer...Continue Reading

References

Jan 1, 1979·Cell and Tissue Research·J Thyberg, S Moskalewski
Oct 1, 1980·Arthritis and Rheumatism·G L Wong
Jan 1, 1982·Calcified Tissue International·H C Tenenbaum, J N Heersche
Jan 1, 1980·Acta Anatomica·P Scott-Savage, B K Hall

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Citations

Sep 1, 1996·Calcified Tissue International·D W Haas, M F Holick
Jun 30, 1994·Biochimica Et Biophysica Acta·E K BasdraA G Papavassiliou
Oct 1, 1987·Collagen and Related Research·G MaorM Silbermann
Feb 1, 2003·Anatomical Science International·Hiroyuki InoueKiyoto Ishizeki
Apr 1, 1991·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·S B MilamD Herbert
Feb 1, 1988·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A WeissM Silbermann
Dec 3, 2016·Journal of Orofacial Orthopedics = Fortschritte Der Kieferorthopädie : Organ/official Journal Deutsche Gesellschaft Für Kieferorthopädie·Konstantinos KaramesinisChristina Piperi
Jul 26, 2005·Journal of Dental Research·G Shen, M Ali Darendeliler
Nov 1, 1990·Journal of Dental Research·F E EngelB D Boyan
May 1, 1991·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·T Kantomaa, B K Hall

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