A tissue-engineered stomach shows presence of proton pump and G-cells in a rat model, resulting in improved anemia following total gastrectomy

Artificial Organs
Tomoyuki MaemuraTakashi Ichikura

Abstract

Despite advances in surgical reconstruction, total gastrectomy still is accompanied by various complications, especially chronic ones, such as pernicious anemia, resulting in refractory malnutrition. As an alternative approach, we have proposed a tissue-engineered stomach as a replacement of the native stomach. This study aimed to assess the secretory functions of a tissue-engineered stomach in a rat model and the nutritional status of the recipients over an extended time period. Stomach epithelial organoid units were isolated from neonatal rats and seeded onto biodegradable polymers. These constructs were implanted into the omenta of adult recipient rats. After 3 weeks, cyst-like structures had formed, henceforth referred to as tissue-engineered stomachs. The recipient stomachs were resected and replaced by their tissue-engineered counterparts. At 24 weeks after implantation, the secretory function of the tissue-engineered stomach was evaluated using immunohistochemical staining. The hemoglobin levels and nutritional status of the recipients were compared with a control group that had undergone a simple Roux-en-Y reconstruction following total gastrectomy. Recipient rats tolerated the tissue-engineered stomachs well. X-ray exa...Continue Reading

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Citations

Mar 28, 2012·Current Opinion in Pediatrics·Daniel E Levin, Tracy C Grikscheit
Feb 28, 2009·Artificial Organs·Paul S Malchesky
Nov 2, 2011·Artificial Organs·Tomoyuki MaemuraDaizoh Saitoh
Jul 11, 2012·World Journal of Surgery·Victor W WongMichael T Longaker
May 25, 2012·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Giuseppe OrlandoRobert J Stratta
Mar 26, 2016·Fibrogenesis & Tissue Repair·Eseelle K HendowRichard M Day
Apr 30, 2013·The Journal of Surgical Research·Tomoyuki MaemuraManabu Kinoshita
Sep 7, 2013·Pediatric Gastroenterology, Hepatology & Nutrition·Sun Hwan Bae
Mar 23, 2017·Cold Spring Harbor Perspectives in Medicine·Andrew Trecartin, Tracy Grikscheit
Apr 19, 2020·Journal of Tissue Engineering and Regenerative Medicine·Caleb D Vogt, Angela Panoskaltsis-Mortari

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