A tissue reconstitution model to study cancer cell-intrinsic and -extrinsic factors in mammary tumourigenesis.

The Journal of Pathology
Bastiaan EversKarin E de Visser

Abstract

The contribution of cancer cell-intrinsic and -extrinsic factors to metastatic breast cancer is still poorly understood, hampering development of novel therapeutic strategies that decrease breast cancer mortality. Cre/loxP-based conditional mouse models of breast cancer present unique opportunities to study sporadic tumour formation and progression in a controlled setting. Unfortunately, the generation of mouse strains carrying multiple mutant alleles needed for such studies is very time-consuming. Moreover, conditional mouse tumour models do not permit independent manipulation of tumour cell-intrinsic and -extrinsic factors. Although the latter can be achieved by cleared fat-pad transplantation of mouse mammary epithelial cells (MMECs) from tumour suppressor gene (TSG) knockouts into wild-type or mutant recipients, this procedure is not possible for mutations that cause embryonic lethality or preclude mammary gland development. Here we show that cleared fat-pad transplantations with MMECs isolated from K14cre;Cdh1(F/F); Trp53(F/F) mice expressing Cre recombinase under control of the cytokeratin-14 promoter and carrying conditional null alleles for p53 and E-cadherin (Cdh1) first resulted in the formation of phenotypically norm...Continue Reading

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Citations

Jan 31, 2015·Proceedings of the National Academy of Sciences of the United States of America·Alec E CerchiariZev J Gartner
Apr 12, 2011·The Journal of Pathology·Metamia CiampricottiKarin E de Visser
Jul 8, 2011·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Ivo J HuijbersJos Jonkers
Sep 4, 2015·Breast Cancer Research : BCR·Marina A GlukhovaMohamed Bentires-Alj
Jun 20, 2015·Cytokine & Growth Factor Reviews·Ka Kui ChanMohamed El-Tanani
Feb 18, 2012·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Sven Rottenberg, Piet Borst
Jun 25, 2010·Nature Reviews. Cancer·Joerg HeyerLynda Chin
Nov 5, 2013·Cold Spring Harbor Protocols·Geulah Livshits, Scott W Lowe

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