PMID: 6409417Jul 1, 1983Paper

A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene

Cell
S D GilliesS Tonegawa

Abstract

We have studied the DNA sequences required for high level expression of a cloned heavy chain immunoglobulin gene stably introduced into mouse myeloma cells by DNA transfection. We found that DNA sequences derived from the germ line JH-C mu region are required for accurate and efficient transcription from a functionally rearranged VH promoter. Similar to viral transcriptional enhancer elements, these cellular sequences stimulate transcription from either the homologous VH gene segment promoter or a heterologous SV40 promoter. They are active when placed on the 5' or 3' side of the rearranged VH gene segment and they function when their orientation is reversed. However, unlike viral enhancers, the Ig gene enhancer appears to act in a tissue-specific manner, since it is active in mouse B cells but not in mouse fibroblasts. The nucleotide sequence of the Ig enhancer region contains repeating elements that closely resemble sequence the possible role of tissue-specific transcription in cell differentiation and malignant transformation.

Citations

Apr 1, 1984·Clinical & Experimental Metastasis·G L Nicolson
Jan 1, 1994·Immunogenetics·C Michard-VanhéeL Pilström
Mar 1, 1990·Current Genetics·S Thompson-Jäger, H Domdey
Jan 1, 1988·Immunogenetics·H J YounP D Gottlieb
Jan 1, 1985·Immunogenetics·F Daniel-VedeleP Kourilsky
Sep 1, 1984·Molecular and Cellular Biochemistry·S Goldstein, R J Shmookler Reis
Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·D M SeverynseM H Edgell
Mar 1, 1985·Journal of Clinical Immunology·K Kelly, U Siebenlist
Jan 1, 1984·Journal of Clinical Immunology·S J Korsmeyer, T A Waldmann
Sep 1, 1991·Journal of Clinical Immunology·H KanekoT Orii
Sep 1, 1985·Somatic Cell and Molecular Genetics·A Van PelT Boon
Jan 1, 1985·Journal of Molecular Evolution·R Nussinov
Jan 1, 1984·Journal of Molecular Evolution·S A JohnsonR J Britten
Apr 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·S Tonegawa
Jul 19, 2013·Plant Molecular Biology·Muhammad Ramzan Khan, Ghulam Muhammad Ali
Jan 1, 1986·Advances in Internal Medicine·M Colb, T G Krontiris
Aug 3, 1987·Journal of Immunological Methods·D D WuR A Phillips
Jul 26, 1991·Journal of Immunological Methods·R W OverellK H Grabstein
Feb 10, 1994·Journal of Immunological Methods·R J Owens, R J Young
Jan 1, 1986·Journal of Steroid Biochemistry·P D DarbreR J King
Aug 1, 1990·Molecular Immunology·B Y Klein, S L Morrison

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