PMID: 9185586May 26, 1997Paper

A tobamovirus genome that contains an internal ribosome entry site functional in vitro

Virology
P A IvanovJ C Atabekov

Abstract

Most eukaryotic mRNAs are translated by a "scanning ribosome" mechanism. We have found that unlike the type member of the genus Tobamovirus, translation of the 3'-proximal coat protein (CP) gene of a crucifer infecting tobamovirus (crTMV) (Dorokhov et al., 1993; 1994) occurred in vitro by an internal ribosome entry mechanism. Three types of synthetic dicistronic RNA transcripts were constructed and translated in vitro: (i) "MP-CP-3'NTR" transcripts contained movement protein (MP) gene, CP gene and the 3'-nontranslated region of crTMV RNA. These constructs were structurally equivalent to dicistronic subgenomic RNAs produced by tobamoviruses in vivo. (ii) "deltaNPT-CP" transcripts contained partially truncated neomycin phosphotransferase I gene and CP gene. (iii) "CP-GUS" transcripts contained the first CP gene and the gene of Escherichia coli beta-glucuronidase (GUS) at the 3'-proximal position. The results indicated that the 148-nt region upstream of the CP gene of crTMV RNA contained an internal ribosome entry site (IRES(CP)) promoting internal initiation of translation in vitro. Dicistronic IRES(CP), containing chimeric mRNAs with the 5'-terminal stem-loop structure preventing translation of the first gene (MP, deltaNPT, or C...Continue Reading

References

Aug 1, 1976·European Journal of Biochemistry·H R Pelham, R J Jackson
Mar 1, 1992·Journal of Virology·K Tsukiyama-KoharaA Nomoto
Jul 1, 1991·The Journal of General Virology·M UgakiM Nishiguchi
Jan 1, 1991·Advances in Virus Research·V I Agol
Sep 5, 1991·Nature·R J Jackson
Dec 1, 1990·Trends in Biochemical Sciences·R J JacksonA Kaminski
Mar 25, 1989·Nucleic Acids Research·T C VerwoerdA Hoekema
Nov 1, 1989·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·N Sonenberg, J Pelletier
Feb 1, 1989·The Journal of Cell Biology·M Kozak
Jan 1, 1986·Advances in Virus Research·M Kozak
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P GoeletJ Karn
Jan 1, 1993·Annual Review of Genetics·E WimmerX Cao
Sep 1, 1993·The Journal of General Virology·R IkedaY Okada
Sep 1, 1996·Microbiological Reviews·G J Belsham, N Sonenberg

❮ Previous
Next ❯

Citations

Dec 21, 2000·The Plant Journal : for Cell and Molecular Biology·P UrwinP M Gilmartin
Sep 8, 2006·RNA·Stephen D BairdMartin Holcik
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yuri L DorokhovJoseph G Atabekov
Jul 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Hannah Miriam JaagDirk Prüfer
Dec 15, 2015·The Plant Journal : for Cell and Molecular Biology·Maria Del Mar Marquès-BuenoGrégory Vert
May 1, 2003·Molecular Plant Pathology·Ulrich Melcher
Dec 20, 2005·Virus Research·Elizabeth L Pettit KnellerW Allen Miller
Dec 21, 2005·Virology·Theo W Dreher, W Allen Miller
Jan 17, 2007·Biochemistry. Biokhimii︠a︡·A M SchwartzJ G Atabekov
Sep 28, 2004·The Journal of General Virology·Tomas CantoPeter Palukaitis
May 30, 2017·Frontiers in Microbiology·Yuri L DorokhovTatiana V Komarova
Jul 21, 2012·Biochemistry. Biokhimii︠a︡·T V KomarovaYu L Dorokhov

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.