A tomato ERF transcription factor, SlERF84, confers enhanced tolerance to drought and salt stress but negatively regulates immunity against Pseudomonas syringae pv. tomato DC3000

Plant Physiology and Biochemistry : PPB
Zhenjun LiQuanhong Yao

Abstract

ERF proteins are plant-specific transcription factors that play significant roles in plant defense against various stresses. However, only little information regarding stress-related ERF genes is available in tomato (Solanum lycopersicum, Sl). In this study, a tomato ERF gene, SlERF84, was cloned and functionally characterized. The nucleus localization of SlERF84-sGFP was confirmed through a transient expression assay. Transactivation assays in yeast demonstrated that SlERF84 functions as a transcriptional activator. Real-time PCR analysis revealed that SlERF84 could be markedly induced by drought, salt and by several phytohormones (ABA, MeJA and ACC). Overexpression of SlERF84 in Arabidopsis endows transgenic plants with ABA hypersensitivity and enhanced tolerance to drought and salt stress. Histochemical staining assay showed that SlERF84 renders transgenic plants better ROS-scavenging capability. Pathogen inoculation assay revealed that SlERF84 might negatively modulate plant defense response to Pseudomonas syringae pv. tomato DC3000. Moreover, the transcript levels of pathogenesis-related genes AtPR1 and AtPR3 were compromised in transgenic Arabidopsis, as compared to that in Col-0 plants when inoculated with Pseudomonas sy...Continue Reading

Citations

Jul 2, 2020·International Journal of Molecular Sciences·Yujie QuKuaifei Xia
Aug 26, 2020·International Journal of Molecular Sciences·Qiushuo SongVijay Joshi
Nov 22, 2019·Frontiers in Plant Science·Zihan ChengTingbo Jiang
Jul 11, 2020·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Charfeddine GharsallahFaten Gorsane
Jan 26, 2021·Plant Science : an International Journal of Experimental Plant Biology·Alessandra FracassoAndrea Carra
Jan 26, 2021·Plant Science : an International Journal of Experimental Plant Biology·Zhenjun LiQuanhong Yao
Mar 27, 2021·Frontiers in Plant Science·Zhenyuan ZangWeiguang Yang

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