A tomato lipase homologous to DAD1 (LeLID1) is induced in post-germinative growing stage and encodes a triacylglycerol lipase

FEBS Letters
Kenji MatsuiTadahiko Kajiwara

Abstract

A tomato lipase gene homologous to Arabidopsis DAD1 (lipase homologous to DAD1; LeLID1) was cloned and characterized. The corresponding transcript increased rapidly during germination of the seeds and reached a maximum level at four days after germination. Thereafter, it decreased rapidly. Little expression could be found in flowers or fruits. Immunoblot analyses showed that the gene products could be found in the cotyledons and hypocotyls, but not in the roots. In the cotyledons most LeLID1 could be recovered in a soluble fraction. The recombinant LeLID1 protein showed maximum lipase activity at pH 8.0. It showed high activity against triacylglycerols (TAGs) with long acyl chains, but little activity with phosphatidylcholine or monogalactosyldiacylglycerol. TAGs composed of short acyl chains could not be a substrate for the enzyme. A possible involvement of LeLID1 in fat mobilization during seed germination is discussed.

References

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Citations

May 1, 2008·Annual Review of Plant Biology·Ian A Graham
Jul 6, 2012·Plant Physiology and Biochemistry : PPB·K R Vijayakumar, Lalitha R Gowda
Mar 28, 2009·Plant Physiology and Biochemistry : PPB·Ana Rita Matos, Anh-Thu Pham-Thi
Jan 27, 2009·Plant Physiology and Biochemistry : PPB·Alina MosblechIngo Heilmann
Jan 13, 2009·Plant Physiology and Biochemistry : PPB·Anne-Laure Quettier, Peter J Eastmond
Nov 27, 2007·The Plant Journal : for Cell and Molecular Biology·Young Sam SeoWoo Taek Kim
Sep 12, 2006·The Plant Journal : for Cell and Molecular Biology·Marianne PoxleitnerJohn C Rogers
Nov 8, 2012·Journal of Experimental Botany·Agnieszka ZienkiewiczAntonio Jesús Castro
Nov 28, 2013·Protein Expression and Purification·Sonali SethSanjukta Patra
Jan 14, 2020·Plant Science : an International Journal of Experimental Plant Biology·Irene DervisiAndreas Roussis

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