A TRACER 3D Co-Culture tumour model for head and neck cancer

Biomaterials
Miki YoungAlison P McGuigan

Abstract

Cancer-associated fibroblasts (CAFs) are a key component of the tumour microenvironment and have been shown to play an important role in the progression of cancer. To probe these tumour-stroma interactions, we incorporated CAFs derived from head and neck cancer patients and squamous carcinoma cells of the hypopharynx (FaDu) into the Tissue Roll for the Analysis of Cellular Environment and Response (TRACER) platform to establish a co-culture platform that simulates the CAF-tumour microenvironmental interactions in head and neck tumours. TRACER culture involves infiltrating cells into a thin fibrous scaffold and then rolling the resulting biocomposite around a mandrel to generate a 3D and layered structure. Patterning the fibrous scaffold biocomposite during fabrication enables control over the specific location of different cell populations in the rolled configuration. Here, we optimized the seeding densities and configurations of the CAF and FaDu cell tissue sections to enable a robust 3D co-culture system under normoxic conditions. Co-culture of CAFs with FaDu cells produced negligible effects on radiation resistance, but did produce increases in proliferation rate and invasive cell migration at 24 and 48 h of culture. Our stu...Continue Reading

Citations

Jan 20, 2018·Advanced Healthcare Materials·Darren RodenhizerAlison P McGuigan
Apr 9, 2020·Cell Communication and Signaling : CCS·Roghayyeh BaghbanPeyman Zare
Aug 8, 2018·International Journal of Molecular Sciences·Sheng ZhuAndreas K Nussler
Aug 15, 2020·Frontiers in Bioengineering and Biotechnology·Chizhu DingXianghua Yan
Aug 10, 2018·Nature Reviews. Genetics·Mukul TewaryPeter W Zandstra
Oct 18, 2019·Journal of Clinical Medicine·Ilya YakavetsLina Bezdetnaya
Jan 14, 2021·Biomaterials Science·Kanishka FernandoEliza Li Shan Fong
Jul 2, 2021·Advanced Drug Delivery Reviews·Matthew L TanClaudia Fischbach
Jul 27, 2021·Frontiers in Cell and Developmental Biology·Annette AffolterNicole Rotter
Jul 30, 2019·Analytical Chemistry·Sabrina M CramerMatthew R Lockett

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