A trans-lentiviral packaging cell line for high-titer conditional self-inactivating HIV-1 vectors

Molecular Therapy : the Journal of the American Society of Gene Therapy
Adam CockrellTal Kafri

Abstract

Lentiviral vector safety has been the impetus underlying the progress in packaging cell line development. The prospects of generating replication-competent lentiviruses (RCLs) and the potential for vector mobilization continue to be the driving force for the advancement of packaging cell lines. We have exploited the trans-lentiviral packaging system to develop the SODk3 packaging cell line for the generation of conditional self-inactivating (cSIN) vectors. Separating the gag-pol genome into two distinct expression cassettes (gag-pro and vpr-RT-IN) may reduce the potential for RCL formation, while concurrently employing cSIN vectors supports retention of the SIN phenotype in target cells and alleviates technical constraints associated with generating producer cell lines. Through development of the SODk3 packaging cell line we determined that the ratio of Gag/Pol in vector particles may be used as an indicator for packaging cell clones that yield high vector titers. Conditional SIN vector titers (1 x 10(7) TU/ml) were augmented through clonal selection. Distinct producer cell clones revealed a parallel between vector titer and transgene expression levels. We exploited this observation to demonstrate that incorporation of an inter...Continue Reading

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Citations

Aug 15, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Thipparat SuwanmaneeTal Kafri
Sep 18, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Matthew BayerTal Kafri
Jan 21, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Janka MátraiThierry VandenDriessche
Sep 22, 2011·Human Gene Therapy·Scott R WittingMadhusudan V Peshwa
Nov 3, 2011·Human Gene Therapy·Sayandip Mukherjee, Adrian J Thrasher
Jan 9, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sophie BroussauBernard Massie
Nov 16, 2010·Future Microbiology·Patrick MaierStephanie Laufs
Jun 19, 2013·Human Gene Therapy Methods·Anna StornaiuoloChiara Bovolenta
Nov 20, 2008·Archives of Virology·Caroline Torne-CelerCorinne Ronfort
Sep 18, 2007·Molecular Biotechnology·Adam S Cockrell, Tal Kafri
Apr 18, 2013·Expert Opinion on Biological Therapy·Maria Mercedes SeguraAlain Garnier
Oct 18, 2011·Neurobiology of Disease·Thomas B LentzR Jude Samulski
Nov 16, 2010·Hematology/oncology Clinics of North America·Arthur Bank
Apr 18, 2012·The Biochemical Journal·Toshie SakumaYasuhiro Ikeda
Apr 26, 2016·Molecular Therapy. Methods & Clinical Development·Otto-Wilhelm MertenChiara Bovolenta
Aug 1, 2015·Molecular Therapy. Methods & Clinical Development·Peirong HuTal Kafri
Jul 14, 2006·BMC Biotechnology·Martine GeraertsRik Gijsbers
Dec 19, 2015·Journal of Cellular Physiology·Kazutaka OuchiHajime Hosoi
Oct 19, 2016·Journal of Occupational and Environmental Hygiene·John HowardPaul Schulte
Dec 9, 2016·Journal of Occupational and Environmental Medicine·Ryan SchlimgenJatin M Vyas
May 30, 2007·The Journal of Gene Medicine·Nadya al YacoubJohn Foerster
Sep 10, 2020·Frontiers in Molecular Neuroscience·Joseph Edward RittinerBoris Kantor
Sep 30, 2010·Experimental Biology and Medicine·Anthony J BellArthur Bank
Jul 21, 2020·Biotechnology Journal·Mariana V FerreiraAna Sofia Coroadinha
Jan 1, 2020·Molecular Therapy. Methods & Clinical Development·Matthew BaulerRobert E Throm
Oct 2, 2021·Molecular Biotechnology·Mathias MangionBruno Gaillet

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