PMID: 7524099Oct 25, 1994Paper

A transcribing RNA polymerase molecule survives DNA replication without aborting its growing RNA chain

Proceedings of the National Academy of Sciences of the United States of America
B LiuB M Alberts

Abstract

We have demonstrated elsewhere that a precisely placed, stalled Escherichia coli RNA polymerase ternary transcription complex (polymerase-RNA-DNA) stays on the DNA template after passage of a DNA replication fork. Moreover, the bypassed complex remains competent to resume elongation of its bound RNA chain. But the simplicity of our experimental system left several important questions unresolved: in particular, might the observation be relevant only to the particular ternary complex that we studied, and can the finding be generalized to a transcribing instead of a stalled RNA polymerase? To address these issues, we have created three additional ternary transcription complexes and examined their fates after passage of a replication fork. In addition, we have examined the fate of moving RNA polymerase molecules during DNA replication. The results suggest that our previous finding applies to all transcription intermediates of the E. coli RNA polymerase.

References

Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·C K SurrattM J Chamberlin
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Citations

Nov 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P Guptasarma
Apr 20, 2013·Chemical Reviews·Hélène GaillardAndrés Aguilera
Nov 21, 2008·Nature·Richard T Pomerantz, Mike O'Donnell
Jan 1, 1997·Annual Review of Biochemistry·S M UptainM J Chamberlin
Apr 2, 2013·Mechanisms of Ageing and Development·Bruce C McKay, Miguel A Cabrita
Jul 2, 1998·Biophysical Journal·L Erijman, R M Clegg
Mar 7, 2014·DNA Repair·Timothy D SilversteinEric C Greene
Sep 12, 2013·Journal of Molecular Biology·Alba DuchFrancesc Posas
Jun 4, 2016·Scientific Reports·Svetlana PetrukAlexander Mazo
Jan 5, 2019·RNA Biology·Zsolt BoldogkőiDóra Tombácz
Sep 15, 2020·Nucleic Acids Research·Jan-Gert Brüning, Kenneth J Marians
Jul 19, 2017·Proceedings of the National Academy of Sciences of the United States of America·Tsuyoshi TerakawaEric C Greene
Jun 15, 2018·Nature Communications·Bo SunMichelle D Wang
Sep 2, 2021·Nucleic Acids Research·Jan-Gert Brüning, Kenneth J Marians
May 17, 1996·Science·A M Deshpande, C S Newlon
Jul 12, 1996·Science·R Landick, J W Roberts

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