A Transcript and Metabolite Atlas of Blackcurrant Fruit Development Highlights Hormonal Regulation and Reveals the Role of Key Transcription Factors

Frontiers in Plant Science
Dorota A JarretRobert D Hancock

Abstract

Blackcurrant fruit collected at six stages of development were assessed for changes in gene expression using custom whole transcriptome microarrays and for variation in metabolite content using a combination of liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry. Principal components analysis demonstrated that fruit development could be clearly defined according to their transcript or metabolite profiles. During early developmental stages, metabolite profiles were dominated by amino acids and tannins, whilst transcript profiles were enriched in functions associated with cell division, anatomical structure morphogenesis and cell wall metabolism. During mid fruit development, fatty acids accumulated and transcript profiles were consistent with seed and embryo development. At the later stages, sugars and anthocyanins accumulated consistent with transcript profiles that were associated with secondary metabolism. Transcript data also indicated active signaling during later stages of fruit development. A targeted analysis of signaling networks revealed a dynamic activation and repression of almost 60 different transcripts encoding transcription factors across the course of fruit development, many of which...Continue Reading

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Citations

Feb 8, 2020·Metabolomics : Official Journal of the Metabolomic Society·Ilka N AbreuChristine A Hackett
Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·J William AllwoodAnita Sønsteby
Apr 5, 2020·Metabolites·Muhammad Maulana Malikul IkramEiichiro Fukusaki
Apr 24, 2019·Journal of Agricultural and Food Chemistry·Ye TianBaoru Yang

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Datasets Mentioned

BETA
E-MTAB-6973

Methods Mentioned

BETA
Feature Extraction
PCA

Software Mentioned

Genespring
geNorm
Trinity
Feature Extraction
AgriGo
eArray
BLASTx
XCALIBUR
Genstat
Term Enrichment Tool

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