PMID: 9419383Feb 21, 1998Paper

A transcription activation system for regulated gene expression in transgenic plants

Proceedings of the National Academy of Sciences of the United States of America
I MooreK Palme

Abstract

A widely applicable promoter system is described that allows a gene of interest to be activated in specific plant tissues after a cross between defined transgenic lines. The promoter, pOp, consists of lac operators cloned upstream of a minimal promoter. No expression was detected from this promoter when placed upstream of a beta-glucuronidase (GUS) reporter gene in transgenic plants. Transcription from the promoter was activated by crossing reporter plants with activator lines that expressed a chimeric transcription factor, LhG4. This factor comprised transcription-activation domain-II from Gal4 of Saccharomyces cerevisiae fused to a mutant lac-repressor that binds its operator with increased affinity. When LhG4 was expressed from the CaMV 35S promoter, the spatial and quantitative expression characteristics of the 35S promoter were exhibited by the GUS reporter. The LhG4/pOp system may be used to study toxic or deleterious gene products, to coordinate the expression of multiple gene products, to restrict transgene phenotypes to the F1 generation, and to generate hybrid seed. The LhG4 system offers spatially regulated gene expression in the tissues of whole plants growing under normal conditions without the need for external in...Continue Reading

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Citations

Jul 18, 2001·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·P Gallois
May 25, 2007·Nature Reviews. Molecular Cell Biology·G Venugopala ReddyElliot M Meyerowitz
Apr 12, 2000·The Plant Journal : for Cell and Molecular Biology·J LoveW F Thompson
Aug 6, 2000·The Plant Journal : for Cell and Molecular Biology·L GälweilerI Moore
Dec 21, 2002·The Plant Journal : for Cell and Molecular Biology·Justin T StegeCarlos F Barbas
Dec 8, 2010·Nucleic Acids Research·Felipe Fenselau de FelippesDetlef Weigel
Aug 3, 2004·Plant Physiology·Nathaniel P Hawker, John L Bowman
Oct 24, 2007·Plant Physiology·David PanikashviliAsaph Aharoni
Dec 7, 2007·Plant Physiology·Mansour KarimiPierre Hilson
Oct 30, 2007·Plant Physiology·Mansour KarimiPierre Hilson
Oct 9, 2009·Plant Physiology·Ana I FernandezPierre Hilson
Dec 16, 2004·The Plant Cell·Ji-Young LeeJohn L Bowman
Jan 3, 2006·The Plant Cell·Tobias WürschumThomas Laux
Jul 16, 2010·The Plant Cell·Rajani SarojamJohn L Bowman
Oct 21, 2010·The Plant Cell·Eilon ShaniNaomi Ori
Jun 9, 2005·BMC Plant Biology·Cawas B EngineerRobert G Kranz
Oct 3, 2013·Journal of Experimental Botany·Anat HendelmanTzahi Arazi
Mar 25, 2014·Plant Science : an International Journal of Experimental Plant Biology·Michael KravchikTzahi Arazi
Jan 1, 2014·Journal of Experimental Botany·Michael KravchikTzahi Arazi
Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·P KachrooD F Klessig

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