PMID: 799244Dec 22, 1976Paper

A transcriptional barrier in the regulatory region of the tryptophan operon of Escherichia coli: its role in the regulation of repressor-independent RNA synthesis

Molecular & General Genetics : MGG
P H Pouwels, H Pannekoek

Abstract

A study was made of the influence of the growth rate of Escherichia coli bacteria on the transcription of the tryptophan (trp) operon, in various trp repressor negative strains. Selective measurement of the levels of RNA transcribed from the regulatory region (reg) of this operon and from the structural genes, respectively, has revealed that the increase of the rate of trpRNA synthesis with bacterial growth rate (Rose and Yanofsky, 1972) is due to a decrease of the frequency of termination of transcription at the transcriptional barrier in the regulatory region of the operon. In a mutant strain of E. coli with a deletion covering the promotor distal part of the regulatory region of the trp operon where the barrier is located, the rate of trpRNA synthesis is not affected by the growth rate. In suA- strains, in which Rho factor activity is reduced the bacterial growth rate does not affect the rate of synthesis of trpRNA. This result suggests that in wild type bacteria Rho factor contributes to the control of the transcription of the trp operon. In bacteria with a mutation rendering Tryptophanyl-tRNA synthetase (TRSase) inactive (trpS- strains) the rate of trpRNA synthesis is affected by the growth rate in the same way as in the i...Continue Reading

References

Jun 15, 1976·Experientia·D Tsuei, H Rouhandeh
Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J W Roberts
Jun 4, 1975·Science·K BertrandC Yanofsky
May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J P RichardsonC Lowery
Feb 28, 1973·Nature: New Biology·B De CrombruggheI Pastan
Jul 15, 1973·Journal of Molecular Biology·N E Murray, W J Brammar
Oct 3, 1973·Nature: New Biology·D McGeochD Morse
Jun 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·S AdhyaB De Crombrugghe
Apr 9, 1974·Molecular & General Genetics : MGG·J DavisonF Brunel
Apr 1, 1968·Journal of Bacteriology·W F Doolittle, C Yanofsky
Dec 20, 1969·Nature·J W Roberts
Jun 16, 1971·Nature: New Biology·M KuwanoD E Morse
Jul 1, 1965·Journal of Molecular Biology·D Gillespie, S Spiegelman
Feb 1, 1966·Journal of Molecular Biology·G Edlin, O Maaloe
Jan 1, 1975·Molecular & General Genetics : MGG·P H Pouwels, J van Rotterdam

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