A transcriptome comparison of time-matched developing human, mouse and rat neural progenitor cells reveals human uniqueness

Toxicology and Applied Pharmacology
Stefan MasjosthusmannEllen Fritsche

Abstract

It is widely accepted that human brain development has unique features that cannot be represented by rodents. Obvious reasons are the evolutionary distance and divergent physiology. This might lead to false predictions when rodents are used for safety or pharmacological efficacy studies. For a better translation of animal-based research to the human situation, human in vitro systems might be useful. In this study, we characterize developing neural progenitor cells from prenatal human and time-matched rat and mouse brains by analyzing the changes in their transcriptome profile during neural differentiation. Moreover, we identify hub molecules that regulate neurodevelopmental processes like migration and differentiation. Consequences of modulation of three of those hubs on these processes were studied in a species-specific context. We found that although the gene expression profiles of the three species largely differ qualitatively and quantitatively, they cluster in similar GO terms like cell migration, gliogenesis, neurogenesis or development of multicellular organism. Pharmacological modulation of the identified hub molecules triggered species-specific cellular responses. This study underlines the importance of understanding s...Continue Reading

Citations

Mar 12, 2019·Current Pharmaceutical Design·Yasamin A JodatSu R Shin
Nov 1, 2018·Molecular Neurobiology·Luan Pereira DinizFlávia Carvalho Alcantara Gomes
Nov 7, 2019·Cell Biology and Toxicology·Miriam E MossobaRobert L Sprando
Jul 10, 2018·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ellen FritscheJulia Tigges
Jul 10, 2018·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ellen FritscheJulia Tigges
Apr 5, 2019·Journal of Veterinary Science·Niroch Nawzad AubidVanessa Jane Hall
May 11, 2021·Cell Biology and Toxicology·Jördis KloseEllen Fritsche
Nov 9, 2021·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Nils FörsterAxel Mosig

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