A Transcriptome-Level Study Identifies Changing Expression Profiles for Ossification of the Ligamentum Flavum of the Spine

Molecular Therapy. Nucleic Acids
Yawei HanYuan Xue

Abstract

Ossification of the ligamentum flavum (OLF) is a common spinal disorder that causes myelopathy and radiculopathy. Non-coding RNAs (ncRNAs) are involved in numerous pathological processes; however, very few ncRNAs have been identified to be correlated with OLF. Here we compared the expression of lncRNA, mRNA, circRNA, and microRNA in OLF tissues from OLF patients and healthy volunteers through mRNA, lncRNA, and circRNA microarrays and microRNA sequencing. A total of 2,054 mRNAs, 2,567 lncRNAs, 627 circRNAs, and 28 microRNAs (miRNAs) were altered during the process of OLF. qPCR confirmed the differential expression of selected mRNAs and ncRNAs. An lncRNA-mRNA co-expression network, miRNA-mRNA target prediction network, and competing endogenous RNA (ceRNA) network of circRNA-miRNA-mRNA were constructed based on a correlation analysis of the differentially expressed RNA transcripts. Subsequently, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses for the differentially expressed mRNAs and the predicted miRNAs target genes were performed. In addition, a deregulated miRNA-19b-3p-based miRNA-circRNA-lncRNA-mRNA network was confirmed, by gain-of-function and loss-of-function experiments, to function ...Continue Reading

Citations

Nov 2, 2018·FEBS Letters·Yawei HanWei Hong
Jul 2, 2019·Molecular Medicine Reports·Daliang KongFei Wang
Dec 31, 2020·Acta Biochimica Et Biophysica Sinica·Zhen ZhangWei Hong
Jan 3, 2021·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Xiaoqiu YuanYu Chen

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Datasets Mentioned

BETA
GSE106256

Methods Mentioned

BETA
transfection
circRNA array
immunoprecipitation
ChIP
transcription profiling
ubiquitination
electrophoresis
Feature Extraction
PCR

Software Mentioned

GeneSpring GX
miRDeep2
Novoalign
limma
Agilent Feature Extraction
Arraystar
R
SPSS

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