A Transcriptomic and Epigenomic Comparison of Fetal and Adult Human Cardiac Fibroblasts Reveals Novel Key Transcription Factors in Adult Cardiac Fibroblasts

JACC. Basic to Translational Science
Malin K B JonssonRoger S Foo

Abstract

Cardiovascular disease remains the number one global cause of death and presents as multiple phenotypes in which the interplay between cardiomyocytes and cardiac fibroblasts (CFs) has become increasingly highlighted. Fetal and adult CFs influence neighboring cardiomyocytes in different ways. Thus far, a detailed comparison between the two is lacking. Using a genome-wide approach, we identified and validated 2 crucial players for maintaining the adult primary human CF phenotype. Knockdown of these factors induced significant phenotypical changes, including senescence and reduced collagen gene expression. These may now represent novel therapeutic targets against deleterious functions of CFs in adult cardiovascular disease.

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Citations

Apr 11, 2019·Tissue Engineering. Part a·Tracy A HookwayTodd C McDevitt
Feb 11, 2020·Annual Review of Physiology·Michelle D Tallquist
Aug 23, 2019·Journal of Cardiovascular Development and Disease·Luis HortellsKatherine E Yutzey
Jul 10, 2018·American Journal of Physiology. Heart and Circulatory Physiology·Momin MohisGracious R Ross
Mar 27, 2021·American Journal of Physiology. Heart and Circulatory Physiology·Alexander J Whitehead, Adam J Engler
Jul 27, 2021·Frontiers in Physiology·Giorgia PalanoErik Müllers
Aug 3, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Martha E FloySean P Palecek
Aug 12, 2021·Npj Regenerative Medicine·Rita N GomesDiana S Nascimento
Aug 28, 2021·International Journal of Molecular Sciences·Charles M KerrYing Mei
Sep 24, 2021·Epigenetics : Official Journal of the DNA Methylation Society·Chaoyang LiXing Fu

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Methods Mentioned

BETA
confocal microscopy
ChIP-seq
flow cytometry
FACS
RNA-seq
immunoprecipitation
SMA
transfection

Software Mentioned

GENCODE
DFilter
TopHat
IPA
Homer
Cufflinks
Ingenuity Pathway Analysis ( IPA
HOMER Motif Analysis
Cuffdiff

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