A transcriptomic approach to identify regulatory genes involved in fruit set of wild-type and parthenocarpic tomato genotypes

Plant Molecular Biology
Fabrizio RuiuA Mazzucato

Abstract

The tomato parthenocarpic fruit (pat) mutation associates a strong competence for parthenocarpy with homeotic transformation of anthers and aberrancy of ovules. To dissect this complex floral phenotype, genes involved in the pollination-independent fruit set of the pat mutant were investigated by microarray analysis using wild-type and mutant ovaries. Normalized expression data were subjected to one-way ANOVA and 2499 differentially expressed genes (DEGs) displaying a >1.5 log-fold change in at least one of the pairwise comparisons analyzed were detected. DEGs were categorized into 20 clusters and clusters classified into five groups representing transcripts with similar expression dynamics. The "regulatory function" group (685 DEGs) contained putative negative or positive fruit set regulators, "pollination-dependent" (411 DEGs) included genes activated by pollination, "fruit growth-related" (815 DEGs) genes activated at early fruit growth. The last groups listed genes with different or similar expression pattern at all stages in the two genotypes. qRT-PCR validation of 20 DEGs plus other four selected genes assessed the high reliability of microarray expression data; the average correlation coefficient for the 20 DEGs was 0.90...Continue Reading

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Citations

Apr 17, 2016·Plant & Cell Physiology·Chonprakun ThagunTsubasa Shoji
Jul 20, 2016·International Journal of Molecular Sciences·Zhaoqun YaoSifeng Zhao
May 3, 2018·Annals of Botany·Bertrand BeauvoitYves Gibon
Nov 13, 2018·The Plant Journal : for Cell and Molecular Biology·Christophe RothanMathilde Causse
Feb 5, 2019·Frontiers in Plant Science·Maurizio E Picarella, Andrea Mazzucato
Jul 12, 2019·Frontiers in Plant Science·Romina PedreschiReinaldo Campos-Vargas
Aug 18, 2017·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Anna PucciAndrea Mazzucato
Jun 8, 2021·Frontiers in Plant Science·Mi HeYanwei Hao
Jun 24, 2021·The Plant Journal : for Cell and Molecular Biology·Estelle BineauMathilde Causse

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