A transcriptomic (RNA-seq) analysis of genes responsive to both cadmium and arsenic stress in rice root

The Science of the Total Environment
Yingmei HuangJicai Yi

Abstract

Cadmium (Cd) and arsenic (As) are nonessential and toxic elements in rice that often occur together in contaminated paddy field soils. To understand whether rice has a common molecular response mechanism against Cd and As toxicity, 30-day seedlings (Oryza sativa L. indica) were exposed separately to Cd and As3+ in hydroponic cultures for up to 7 days. Root transcriptomic analysis of plants exposed to Cd and As for 3 days revealed that a total of 2224 genes in rice roots responded to Cd stress, while 1503 genes responded to As stress. Of these, 841 genes responded to both stressors. The genes in common to Cd and As stress were associated with redox control, stress response, transcriptional regulation, transmembrane transport, signal transduction, as well as biosynthesis and metabolism of macromolecules and sulfur compounds. In plants exposed to Cd and As separately or in combination for 3 and 7 days, qRT-PCR verification revealed that the glutathione metabolism associated gene Os09g0367700 was significantly up-regulated with respect to unexposed controls and had a positive synergistic effect under combined Cd and As stress. In addition, the redox control related genes Os06g0216000, Os07g0638300 and Os01g0294500, the glutathione ...Continue Reading

Citations

Apr 4, 2021·Plants·Paola I Angulo-BejaranoRocío Cruz-Ortega
Jul 31, 2021·Journal of Hazardous Materials·Keji WangTingxuan Li
Apr 21, 2020·Journal of Agricultural and Food Chemistry·Yucheng LiHuining Xiao

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