A transcriptomics resource reveals a transcriptional transition during ordered sarcomere morphogenesis in flight muscle

ELife
Maria L SpletterFrank Schnorrer

Abstract

Muscles organise pseudo-crystalline arrays of actin, myosin and titin filaments to build force-producing sarcomeres. To study sarcomerogenesis, we have generated a transcriptomics resource of developing Drosophila flight muscles and identified 40 distinct expression profile clusters. Strikingly, most sarcomeric components group in two clusters, which are strongly induced after all myofibrils have been assembled, indicating a transcriptional transition during myofibrillogenesis. Following myofibril assembly, many short sarcomeres are added to each myofibril. Subsequently, all sarcomeres mature, reaching 1.5 µm diameter and 3.2 µm length and acquiring stretch-sensitivity. The efficient induction of the transcriptional transition during myofibrillogenesis, including the transcriptional boost of sarcomeric components, requires in part the transcriptional regulator Spalt major. As a consequence of Spalt knock-down, sarcomere maturation is defective and fibers fail to gain stretch-sensitivity. Together, this defines an ordered sarcomere morphogenesis process under precise transcriptional control - a concept that may also apply to vertebrate muscle or heart development.

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Citations

Mar 16, 2019·Development·Ashley A DeAgueroRichard M Cripps
Dec 10, 2019·The Journal of Cell Biology·Szilárd SzikoraJózsef Mihály
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Feb 20, 2020·Seminars in Cell & Developmental Biology·Elena NikonovaMaria L Spletter

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Datasets Mentioned

BETA
GSE107247

Methods Mentioned

BETA
confocal microscopy
PCR
RNA-Seq

Software Mentioned

ComplexHeatmap
GO
RColorBrewer
featureCounts
Photoshop
MyofibrilJ
pStinger
Excel
DESeq2
Fiji J

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