A transgene design for enhancing oil content in Arabidopsis and Camelina seeds

Biotechnology for Biofuels
Yerong ZhuHenrik Vibe Scheller

Abstract

Increasing the oil yield is a major objective for oilseed crop improvement. Oil biosynthesis and accumulation are influenced by multiple genes involved in embryo and seed development. Theleafy cotyledon1(LEC1) is a master regulator of embryo development that also enhances the expression of genes involved in fatty acid biosynthesis. We speculated that seed oil could be increased by targeted overexpression of a master regulating transcription factor for oil biosynthesis, using a downstream promoter for a gene in the oil biosynthesis pathway. To verify the effect of such a combination on seed oil content, we made constructs with maize (Zea mays)ZmLEC1driven by serine carboxypeptidase-like (SCPL17) and acyl carrier protein (ACP5)promoters, respectively, for expression in transgenicArabidopsis thalianaandCamelina sativa. Agrobacterium-mediated transformation successfully generated Arabidopsis and Camelina lines that overexpressedZmLEC1under the control of a seed-specific promoter. This overexpression does not appear to be detrimental to seed vigor under laboratory conditions and did not cause observable abnormal growth phenotypes throughout the life cycle of the plants. Overexpression ofZmLEC1increased the oil content in mature seed...Continue Reading

References

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Citations

Jan 4, 2019·The Plant Journal : for Cell and Molecular Biology·GunNam NaChaofu Lu
Jun 10, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Kevin KingChaofu Lu
Feb 14, 2019·BMC Plant Biology·Solomon H Stonebloom, Henrik Vibe Scheller

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Methods Mentioned

BETA
transgenic
PCR
nuclear magnetic resonance
reverse transcription-PCR
NMR
reverse
PCRs

Software Mentioned

ATTED
IDT DNA Real - Time
GraphPad
XLSTAT

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