A transgenic mouse model with inducible Tyrosinase gene expression using the tetracycline (Tet-on) system allows regulated rescue of abnormal chiasmatic projections found in albinism

Pigment Cell Research
Estela GiménezL Montoliu

Abstract

Congenital defects in retinal pigmentation, as in oculocutaneous albinism Type I (OCA1), where tyrosinase is defective, result in visual abnormalities affecting the retina and pathways into the brain. Transgenic animals expressing a functional tyrosinase gene on an albino genetic background display a correction of all these abnormalities, implicating a functional role for tyrosinase in normal retinal development. To address the function of tyrosinase in the development of the mammalian visual system, we have generated a transgenic mouse model with inducible expression of the tyrosinase gene using the tetracycline (TET-ON) system. We have produced two types of transgenic mice: first, mice expressing the transactivator rtTA chimeric protein under the control of mouse tyrosinase promoter and its locus control region (LCR), and; second, transgenic mice expressing a mouse tyrosinase cDNA construct driven by a minimal promoter inducible by rtTA in the presence of doxycycline. Inducible experiments have been carried out with selected double transgenic mouse lines. Tyrosinase expression has been induced from early embryo development and its impact assessed with histological and biochemical methods in heterozygous and homozygous double ...Continue Reading

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Citations

Oct 7, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Zetang WuDavid J Fink
Apr 14, 2005·BMC Cell Biology·Alfonso LavadoLluís Montoliu
Apr 2, 2005·The Journal of Comparative Neurology·Estela GiménezLluís Montoliu
Jul 23, 2011·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Hui YaoShaorong Gao
Dec 13, 2005·Genesis : the Journal of Genetics and Development·Aubin MichalonIsabelle M Mansuy
Jun 12, 2008·Developmental Biology·Alexander Emelyanov, Serguei Parinov
May 16, 2018·Journal of Neuroscience Research·Brian S McKay
Dec 28, 2018·Investigative Ophthalmology & Visual Science·Helen T MichaelChi-Ping Day
Oct 25, 2020·Pigment Cell & Melanoma Research·Davide SeruggiaLluis Montoliu
Nov 2, 2020·Survey of Ophthalmology·Siyin LiuMervyn G Thomas

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