Oct 30, 2018

A transgenic zebrafish line for in vivo visualisation of neutrophil myeloperoxidase

BioRxiv : the Preprint Server for Biology
Kyle D BuchanStephen A Renshaw

Abstract

The neutrophil enzyme myeloperoxidase (MPO) is a major enzyme made by neutrophils to generate antimicrobial and immunomodulatory compounds, notably hypochlorous acid (HOCl), amplifying their capacity for destroying pathogens and regulating inflammation. Despite its roles in innate immunity, the importance of MPO in preventing infection is unclear, as individuals with MPO deficiency are asymptomatic with the exception of an increased risk of candidiasis. Dysregulation of MPO activity is also linked with inflammatory conditions such as atherosclerosis, emphasising a need to understand the roles of the enzyme in greater detail. Consequently, new tools for investigating granular dynamics in vivo can provide useful insights into how MPO localises within neutrophils, aiding understanding of its role in preventing and exacerbating disease. The zebrafish is a powerful model for investigating the immune system in vivo, as it is genetically tractable, and optically transparent. To visualise MPO activity within zebrafish neutrophils, we created a genetic construct that expresses human MPO as a fusion protein with a C-terminal fluorescent tag, driven by the neutrophil-specific promoter lyz. After introducing the construct into the zebrafis...Continue Reading

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Mentioned in this Paper

Establishment and Maintenance of Localization
Fluorescent Dyes
Study
In Vivo
Immune System
MRNA Maturation
Carboxy-Terminal Amino Acid
Genome
Neutrophil Migration, Function
Neutrophils as Percentage of Blood Leukocytes (Lab Test)

About this Paper

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