A transglucosidase necessary for starch degradation and maltose metabolism in leaves at night acts on cytosolic heteroglycans (SHG)

The Plant Journal : for Cell and Molecular Biology
Joerg FettkeMartin Steup

Abstract

The recently characterized cytosolic transglucosidase DPE2 (EC 2.4.1.25) is essential for the cytosolic metabolism of maltose, an intermediate on the pathway by which starch is converted to sucrose at night. In in vitro assays, the enzyme utilizes glycogen as a glucosyl acceptor but the in vivo acceptor molecules remained unknown. In this communication we present evidence that DPE2 acts on the recently identified cytosolic water-soluble heteroglycans (SHG) as does the cytosolic phosphorylase (EC 2.4.1.1) isoform. By using in vitro two-step (14)C labeling assays we demonstrate that the two transferases can utilize the same acceptor sites of the SHG. Cytosolic heteroglycans from a DPE2-deficient Arabidopsis mutant were characterized. Compared with the wild type the glucose content of the heteroglycans was increased. Most of the additional glucosyl residues were found in the outer chains of SHG that are released by an endo-alpha-arabinanase (EC 3.2.1.99). Additional starch-related mutants were characterized for further analysis of the increased glucosyl content. Based on these data, the cytosolic metabolism of starch-derived carbohydrates is discussed.

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Citations

May 24, 2008·The Journal of Biological Chemistry·Jon M SteichenThomas D Sharkey
Mar 28, 2009·Journal of Experimental Botany·Joerg FettkeMartin Steup
Sep 18, 2013·Plant Physiology·Marina Camara Mattos MartinsJohn Edward Lunn
Feb 9, 2013·The Arabidopsis Book·Sebastian Streb, Samuel C Zeeman
Jan 29, 2011·Journal of Plant Physiology·Irina MalinovaJoerg Fettke
Nov 23, 2010·Journal of Plant Physiology·Joerg FettkeMartin Steup
Apr 28, 2009·Phytochemistry·Joerg FettkeMartin Steup
Apr 17, 2007·Current Opinion in Plant Biology·Anna LytovchenkoAlisdair R Fernie
Jul 31, 2007·Plant, Cell & Environment·Alison M Smith, Mark Stitt
Oct 27, 2011·Molecular Systems Biology·Onder KartalOliver Ebenhöh
May 27, 2015·Trends in Plant Science·Joerg Fettke, Alisdair R Fernie
Aug 21, 2013·Journal of Experimental Botany·Lenka Franková, Stephen C Fry
May 21, 2011·Plant Signaling & Behavior·Irina MalinovaJoerg Fettke
Aug 29, 2006·Plant & Cell Physiology·Jacob G MaloneNicholas J Kruger
Apr 23, 2010·The Plant Journal : for Cell and Molecular Biology·Mark StittBjörn Usadel
Feb 23, 2020·Annual Review of Plant Biology·Alison M Smith, Samuel C Zeeman
Jun 21, 2018·Frontiers in Plant Science·Irina MalinovaJoerg Fettke
Jun 1, 2007·Functional Plant Biology : FPB·Samuel C ZeemanOliver Kötting
May 30, 2021·The Plant Journal : for Cell and Molecular Biology·Angel Mérida, Joerg Fettke
May 31, 2021·The New Phytologist·Américo J C VianaMichel Vincentz
Jul 17, 2012·Carbohydrate Research·Krit TantanaratRobert A Field

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