Aug 21, 2015

A transient ischemic environment induces reversible compaction of chromatin

BioRxiv : the Preprint Server for Biology
Ina KirmesGeorge Reid


The environmental effects of ischemia on chromatin nanostructure were evaluated using single molecule localisation microscopy (SMLM) of DNA binding dyes. Short-term oxygen and nutrient deprivation (OND) of the cardiomyocyte cell-line HL-1induces a previously undescribed chromatin architecture, consisting of large, chromatin sparse voids interspersed between DNA-dense hollow helicoid structures of the order of 40 to 700 nm in dimension. OND induced chromatin compaction is reversible, and upon restitution of normoxia and nutrients, chromatin transiently adopts a significantly more open structure than in untreated cells. We show that this compacted state of chromatin reduces transcription, while the open chromatin structure following recovery has a higher transcriptional rate than in untreated cells. Digestion of chromatin with DNAseI and DNA binding dye loading assays confirm that OND induces compaction of chromatin and a general redistribution of chromatin to the nuclear periphery. Mechanistically, chromatin compaction is associated with a depletion of intracellular ATP and a redistribution of the cellular polyamine pool into the nucleus. Additionally, Fluorescence Recovery After Photobleaching (FRAP) shows that core histones ar...Continue Reading

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Mentioned in this Paper

Establishment and Maintenance of Localization
Antiandrogen Therapy
Transcription, Genetic
FRAP1 protein, human
SPT6 Protein

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