Aug 21, 2015

A transient ischemic environment induces reversible compaction of chromatin

BioRxiv : the Preprint Server for Biology
Ina KirmesGeorge Reid

Abstract

The environmental effects of ischemia on chromatin nanostructure were evaluated using single molecule localisation microscopy (SMLM) of DNA binding dyes. Short-term oxygen and nutrient deprivation (OND) of the cardiomyocyte cell-line HL-1induces a previously undescribed chromatin architecture, consisting of large, chromatin sparse voids interspersed between DNA-dense hollow helicoid structures of the order of 40 to 700 nm in dimension. OND induced chromatin compaction is reversible, and upon restitution of normoxia and nutrients, chromatin transiently adopts a significantly more open structure than in untreated cells. We show that this compacted state of chromatin reduces transcription, while the open chromatin structure following recovery has a higher transcriptional rate than in untreated cells. Digestion of chromatin with DNAseI and DNA binding dye loading assays confirm that OND induces compaction of chromatin and a general redistribution of chromatin to the nuclear periphery. Mechanistically, chromatin compaction is associated with a depletion of intracellular ATP and a redistribution of the cellular polyamine pool into the nucleus. Additionally, Fluorescence Recovery After Photobleaching (FRAP) shows that core histones ar...Continue Reading

  • References
  • Citations

References

  • We're still populating references for this paper, please check back later.
  • References
  • Citations

Citations

  • This paper may not have been cited yet.

Mentioned in this Paper

Establishment and Maintenance of Localization
Ischemia
Study
Antiandrogen Therapy
Protoplasm
Transcription, Genetic
Environment
FRAP1 protein, human
Nutrients
SPT6 Protein

About this Paper

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.