A translational enhancer element on the 3'-proximal end of the Panicum mosaic virus genome

FEBS Letters
Jeffrey S BattenKaren-Beth G Scholthof

Abstract

Panicum mosaic virus (PMV) is a single-stranded positive-sense RNA virus in the family Tombusviridae. PMV genomic RNA (gRNA) and subgenomic RNA (sgRNA) are not capped or polyadenylated. We have determined that PMV uses a cap-independent mechanism of translation. A 116-nucleotide translational enhancer (TE) region on the 3'-untranslated region of both the gRNA and sgRNA has been identified. The TE is required for efficient translation of viral proteins in vitro. For mutants with a compromised TE, addition of cap analog, or transposition of the cis-active TE to another location, both restored translational competence of the 5'-proximal sgRNA genes in vitro.

References

Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·M Kozak
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·M Kozak
Oct 1, 1996·Plant Molecular Biology·D R Gallie
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
Dec 21, 2005·Virology·Theo W Dreher, W Allen Miller
Jul 1, 2002·Molecular Plant Pathology·W Allen MillerRandy Beckett

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Citations

May 21, 2013·Annual Review of Microbiology·Anne E Simon, W Allen Miller
May 29, 2010·RNA·Beth L NicholsonK Andrew White
Mar 24, 2012·Current Opinion in Virology·Beth L Nicholson, K Andrew White
Oct 29, 2010·Virus Research·Aurora CastañoCarmen Hernández
Sep 9, 2008·Virology·Beth L Nicholson, K Andrew White
Feb 11, 2015·Virus Research·Anne E Simon
Dec 15, 2017·Frontiers in Plant Science·Verónica TrunigerMiguel A Aranda
Apr 22, 2017·Frontiers in Plant Science·Manuel MirasMiguel A Aranda
Dec 6, 2018·Nature Reviews. Microbiology·Zane A Jaafar, Jeffrey S Kieft

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