PMID: 7947917Nov 2, 1994

A transmembrane potential does not affect the vertical location of charged lipid spin labels with respect to the surface of a phosphatidylcholine bilayer

Biochimica Et Biophysica Acta
E Jo, J M Boggs


The effect of a transmembrane potential on the vertical location of a charged lipid in a neutral phosphatidylcholine (PC) lipid bilayer has been investigated using negatively and positively charged spin-labeled lipids. A transmembrane potential was generated across extruded large unilamellar vesicles either by using a K+/Na+ ion gradient and a K+ ionophore or by using a pH gradient. Since a transmembrane potential could have opposing effects on lipids in the inner and outer monolayer, some of the acidic spin labels were asymmetrically located in the inner monolayer as a result of a pH gradient. No significant effect on their order parameters was observed upon applying a transmembrane potential. The internal dipole potential of the bilayer was modified by using dialkyl-PC or by incorporating 10 mol% phloretin, or 6-ketocholestanol in the PC, but a transmembrane potential still had no detectable effect on the spin labeled lipids. Therefore, it is concluded that the electrochemical potential across membranes probably does not cause a significant change in the vertical location of charged lipids with respect to the surface of a PC bilayer. This suggests that polar interactions and/or van der Waals interactions between the spin prob...Continue Reading


Nov 1, 1976·Chemistry and Physics of Lipids·A SansonC M Gary-Bobo
Feb 6, 1978·The Journal of Membrane Biology·J M Boggs, M A Moscarello
Sep 27, 1979·Biochemical and Biophysical Research Communications·P I Lelkes
Mar 17, 1981·Biochemistry·J Teissie, T Y Tsong
Jan 1, 1982·The Journal of Membrane Biology·J N WeinsteinR D Klausner


Aug 19, 1997·Proceedings of the National Academy of Sciences of the United States of America·C HertelJ A Kemp

Related Concepts

Electron Spin Resonance Spectroscopy
Lipid Bilayers
Study of Magnetics
Resting Potentials

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