A trehalose biosynthetic enzyme doubles as an osmotic stress sensor to regulate bacterial morphogenesis

PLoS Genetics
Xi-Ming ChenPaul Dyson

Abstract

The dissacharide trehalose is an important intracellular osmoprotectant and the OtsA/B pathway is the principal pathway for trehalose biosynthesis in a wide range of bacterial species. Scaffolding proteins and other cytoskeletal elements play an essential role in morphogenetic processes in bacteria. Here we describe how OtsA, in addition to its role in trehalose biosynthesis, functions as an osmotic stress sensor to regulate cell morphology in Arthrobacter strain A3. In response to osmotic stress, this and other Arthrobacter species undergo a transition from bacillary to myceloid growth. An otsA null mutant exhibits constitutive myceloid growth. Osmotic stress leads to a depletion of trehalose-6-phosphate, the product of the OtsA enzyme, and experimental depletion of this metabolite also leads to constitutive myceloid growth independent of OtsA function. In vitro analyses indicate that OtsA can self-assemble into protein networks, promoted by trehalose-6-phosphate, a property that is not shared by the equivalent enzyme from E. coli, despite the latter's enzymatic activity when expressed in Arthrobacter. This, and the localization of the protein in non-stressed cells at the mid-cell and poles, indicates that OtsA from Arthrobact...Continue Reading

References

Jun 1, 1992·The Journal of Applied Bacteriology·C E Deutch, G S Perera
Jun 1, 1990·Journal of Bacteriology·W BoosP Postma
Dec 1, 1980·Journal of Bacteriology·J J Germida, L E Casida
Jan 1, 1995·Trends in Biochemical Sciences·J M Thevelein, S Hohmann
May 1, 1994·Journal of Bacteriology·A Mukherjee, J Lutkenhaus
Oct 8, 1999·Antonie van Leeuwenhoek·S Malwane, C E Deutch
Feb 5, 2000·Microbiology·K A De SmetB D Robertson
Jan 25, 2002·Acta Crystallographica. Section D, Biological Crystallography·Robert P GibsonGideon J Davies
Mar 11, 2003·Glycobiology·Alan D ElbeinDavid Carroll
Aug 13, 2004·Molecular Plant-microbe Interactions : MPMI·Claudia B Monteiro-VitorelloJoão C Setúbal
Oct 18, 2005·Nature Reviews. Molecular Cell Biology·William Margolin
Feb 18, 2006·Journal of Bacteriology·Ashwini ChauhanMalini Rajagopalan
Jul 31, 2007·Cell·Richard B WeartPetra Anne Levin
Dec 25, 2007·Nature Reviews. Microbiology·Sheryl S JusticeScott J Hultgren
Mar 7, 2009·Micron : the International Research and Review Journal for Microscopy·A PianettiF Bruscolini
Aug 15, 2009·Nature Reviews. Microbiology·David W Adams, Jeff Errington
Nov 1, 2004·FEMS Microbiology Ecology·Héctor M AlvarezAlexander Steinbüchel
Feb 16, 2010·Developmental Cell·Sunish Kumar RadhakrishnanPatrick H Viollier
Mar 13, 2010·The Journal of Biological Chemistry·David PoppRobert C Robinson
Jun 2, 2010·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Ji-Long Liu
Jul 20, 2010·Nature Cell Biology·Michael Ingerson-MaharZemer Gitai
Aug 18, 2010·The Journal of Cell Biology·Chalongrat NoreeJames E Wilhelm
Jan 20, 2011·Molecular Microbiology·Eva-Maria KleinschnitzGünther Muth
Feb 22, 2011·Cell·Gyanendra P Dubey, Sigal Ben-Yehuda
Jun 2, 2011·Extremophiles : Life Under Extreme Conditions·Xi-Ming ChenLi-Zhe An
Jun 4, 2011·Science·Julia Domínguez-EscobarRut Carballido-López
Aug 13, 2013·PLoS Genetics·Norbert S HillPetra Anne Levin

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Methods Mentioned

BETA
salt stress
scanning electron microscopy
Fluorescence microscopy
dynamic light scattering
Transmission electron microscopy
Fluorescence
coimmunoprecipitation
electron microscopy

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