A Tri-fusion Reporter Mouse Reveals Tissue-Specific FGF1B Promoter Activity in vivo

Scientific Reports
Shan-Wen LiuKurt M Lin

Abstract

Transgenic mice harboring imaging reporters take full advantage of imaging technologies in studies using living mice. Here, we established a tri-fusion multimodal reporter gene containing fragments from firefly luciferase, enhanced green fluorescent protein, and herpes simplex virus type 1 thymidine kinase and generated tri-fusion reporter Tg mice. Fibroblast growth factor type 1 (FGF1), a multifunctional mitogen to a wide range of tissues, regulates proliferation of neural stem cells of the brain, where FGF1 expression is initiated through activation of the FGF1B (F1B) promoter. The reporter mouse under the control of the human F1B promoter enables visualization in vivo where F1B activity is elevated, including tissues not only in the brain but also in the nasopharynx, skull, spine, and testes, particularly in Leydig cells. Treating Tg mice with the alkylating agent busulfan, which is known to eradicate Leydig cells and disrupt spermatogenesis in mice, eliminated the reporter signals. Restoring Leydig cells recovered reporter expression, indicating that the reporter can be used as a surrogate marker for Leydig cells. The F1B tri-fusion reporter mouse model can be utilized in longitudinal monitoring of the health status of the ...Continue Reading

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Citations

Nov 5, 2020·International Journal of Molecular Sciences·Tzu-Yin ChenKurt Ming-Chao Lin

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Datasets Mentioned

BETA
CAA23742

Methods Mentioned

BETA
transgenic
PCR
fluorescence imaging
fluorescence
flow cytometry
fluorescence-activated cell sorting
confocal microscopy
genotyping
electrophoresis

Software Mentioned

AMIDE

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