A tri-modal molecular imaging agent for sentinel lymph node mapping

Nuclear Medicine and Biology
Zhengtao QinDavid R Vera

Abstract

We report an "instant kit" method to radiolabel fluorescent-tilmanocept with (68)Ga and (99m)Tc for tri-modal molecular imaging of sentinel lymph nodes (SLNs). Solutions of sodium acetate, (68)GaCl(3) and Na(99m)TcO(4) were added successively to a "kit vial" containing lyophilized 800CW-tilmanocept, SnCl(2), trehalose and ascorbic acid. After a 30-min incubation, the pH was neutralized with PBS. No purification was required. Radiochemical and fluorescence purity was measured by HPLC and ITLC techniques. In vitro stability was measured by standing gel chromatography (SGC) and ITLC by a 100-fold dilution 0.25 h after radiolabeling. In vivo stability was measured by SGC and ITLC after an 11h incubation in human plasma. A dose (0.1 nmol, ~1MBq (68)Ga, ~25M Bq (99m)Tc) was injected to the footpad of 4 mice. Popliteal SLNs were imaged by PET and fluorescence imaging systems at 0.5, 24, 48, 72 h, then excised and assayed for (99m)Tc. Radiochemical and fluorescent purity exceeded 98%. The in vitro stability assay demonstrated high irreversibility of both radiolabels and the fluorescent label, and in vivo stability assay demonstrated high stability of the technetium and fluorescent labels to plasma metabolism. Popliteal SLNs were identi...Continue Reading

References

Aug 8, 2001·Advanced Drug Delivery Reviews·M A Swartz
Dec 6, 2006·Annals of Surgical Oncology·Anne M WallaceDavid R Vera
Jun 14, 2014·The Journal of Surgical Research·Ava HosseiniDavid R Vera

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Citations

Aug 15, 2017·Clinical and Translational Imaging : Reviews in Nuclear Medicine and Molecular Imaging·S L BugbyA C Perkins
Nov 8, 2020·European Journal of Nuclear Medicine and Molecular Imaging·Rutger MahieuBart de Keizer
Dec 29, 2020·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Remco de BreeAlfio Ferlito
Feb 24, 2019·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Zhengtao QinDavid R Vera
Feb 6, 2017·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Hak J LeeChristopher J Kane

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