A trial of immunotherapy against Leishmania amazonensis infection in vitro and in vivo with Z-100, a polysaccharide obtained from Mycobacterium tuberculosis, alone or combined with meglumine antimoniate

The Journal of Antimicrobial Chemotherapy
Paola Andrea BarrosoYoshihisa Hashiguchi

Abstract

To determine the efficacy and the immunomodulatory function of Z-100 alone or combined with meglumine antimoniate on Leishmania amazonensis infection. The effect of the compounds was evaluated by microscopic counting of intracellular amastigotes in macrophages stained with Giemsa, or axenic promastigotes, and IC(50) was determined by linear regression. The antileishmanial effect of the compounds was assessed in infected BALB/c mice by a limiting dilution analysis and the production of gamma interferon (IFN-gamma), interleukin 10 (IL-10), IL-4, IgG1 and IgG2a was measured by ELISA. In vitro, Z-100 showed antileishmanial activity against intracellular amastigotes of L. amazonensis with an IC(50) of 13 mg/L. Moreover, infected macrophages treated with Z-100 (12 mg/L) showed smaller parasitophorous vacuoles with fewer parasites than the control. In addition, the efficacy of Z-100 plus meglumine antimoniate [14 mg/L pentavalent antimony (Sb(v))] was higher (46% inhibition) than either Z-100 or meglumine antimoniate alone. Nevertheless, no effect of Z-100 on axenic promastigotes was observed. Infected BALB/c mice treated with Z-100 (100 microg/kg) alone did not show any antileishmanial effects in comparison with the control group, an...Continue Reading

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Citations

Aug 21, 2013·The Journal of Antimicrobial Chemotherapy·Kevin J PeineEric M Bachelder
Sep 10, 2013·Research in Veterinary Science·D B BentoP R Romão
Aug 1, 2014·Expert Opinion on Biological Therapy·Ali Khamesipour
Dec 14, 2011·Archives of Medical Research·Fatemeh DehghaniNariman Mossaffa
Dec 17, 2014·European Journal of Medicinal Chemistry·Alex Evangelista do AmaralGuilhermina Rodrigues Noleto
Aug 31, 2011·International Immunopharmacology·Piu SahaMitali Chatterjee
Oct 25, 2016·PLoS Neglected Tropical Diseases·Mohammad IslamuddinFarhat Afrin
Dec 16, 2016·Parasitology·Y TaslimiS Rafati
Dec 21, 2011·Revista Do Instituto De Medicina Tropical De São Paulo·Diana Copi AyresSelma Giorgio
Dec 13, 2016·Transactions of the Royal Society of Tropical Medicine and Hygiene·Anuradha Ratna, Sunil K Arora
Dec 19, 2019·Microorganisms·Camila Dos Santos Meira, Lashitew Gedamu
Oct 23, 2009·The Journal of Physical Chemistry. B·Katarzyna Hac-WydroRadosław Zuk
Jul 15, 2015·International Journal of Biological Macromolecules·Monica Mendes Kangussu-MarcolinoGuilhermina Rodrigues Noleto

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