A Trimodal Imaging Platform for Tracking Viable Transplanted Pancreatic Islets In Vivo: F-19 MR, Fluorescence, and Bioluminescence Imaging

Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging
A GálisováD Jirák

Abstract

Combining specific and quantitative F-19 magnetic resonance imaging (MRI) with sensitive and convenient optical imaging provides complementary information about the distribution and viability of transplanted pancreatic islet grafts. In this study, pancreatic islets (PIs) were labeled with positively charged multimodal nanoparticles based on poly(lactic-co-glycolic acid) (PLGA-NPs) with encapsulated perfluoro-15-crown-5-ether and the near-infrared fluorescent dye indocyanine green. One thousand and three thousand bioluminescent PIs were transplanted into subcutaneous artificial scaffolds, which served as an alternative transplant site. The grafts were monitored using in vivo F-19 MR, fluorescence, and bioluminescence imaging in healthy rats for 2 weeks. Transplanted PIs were unambiguously localized in the scaffolds by F-19 MRI throughout the whole experiment. Fluorescence was detected in the first 4 days after transplantation only. Importantly, in vivo bioluminescence correlated with the F-19 MRI signal. We developed a trimodal imaging platform for in vivo examination of transplanted PIs. Fluorescence imaging revealed instability of the fluorescent dye and its limited applicability for longitudinal in vivo studies. A correlation...Continue Reading

References

Oct 1, 1985·Transplantation·M GotohA P Monaco
Mar 10, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yuxin LuDaniel L Kaufman
Nov 25, 2004·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Daniel JirákMilan Hájek
Sep 28, 2007·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Mangala SrinivasEric T Ahrens
Feb 26, 2008·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·C TosoT Berney
Apr 30, 2010·Trends in Biotechnology·Mangala SrinivasI Jolanda M de Vries
Sep 15, 2010·International Journal of Cancer. Journal International Du Cancer·Fernando BonettoI Jolanda M de Vries
Jan 5, 2011·Transplantation·Frantisek SaudekMilan Hájek
Jul 27, 2011·Current Diabetes Reports·Elisa Cantarelli, Lorenzo Piemonti
Aug 24, 2011·Contrast Media & Molecular Imaging·Brad P BarnettJeff W M Bulte
Jan 5, 2012·PloS One·Philipp Boehm-SturmTherése Kallur
Feb 23, 2012·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Sebastian TemmeUlrich Flögel
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Sep 11, 2012·Biomaterials·Mangala SrinivasMathias Hoehn
Sep 23, 2014·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Eric T AhrensClaudiu Schirda
Mar 15, 2015·PloS One·Jeffrey M GaudetPaula J Foster
Mar 17, 2015·Nuclear Medicine and Molecular Imaging·Jung Eun KimByeong-Cheol Ahn
Jul 5, 2017·Biological Procedures Online·Vít HerynekDaniel Jirák
Jan 1, 2007·Cell Transplantation·Juliet A Emamaullee, A M James Shapiro
Feb 15, 2018·Contrast Media & Molecular Imaging·Andrea GálisováDaniel Jirák
Apr 20, 2018·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Sayuan LiangUwe Himmelreich

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Citations

May 8, 2020·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·M MousavinejadC J Hill
Dec 1, 2018·Magma·Daniel JirakMartin Hruby
Oct 17, 2020·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Lei ZhengYulian Wu
Feb 19, 2021·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Zuzana HladíkováFrantišek Saudek
Jul 31, 2020·Molecular Therapy. Methods & Clinical Development·Chung-Man MoonSang-Soo Shin
Mar 20, 2021·Frontiers in Endocrinology·Dian R Arifin, Jeff W M Bulte
May 5, 2021·Cancer Immunology Research·Zebin Xiao, Ellen Puré
Oct 28, 2021·Chemistry : a European Journal·Dawid Janasik, Tomasz Krawczyk

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Methods Mentioned

BETA
genetic modification
light scattering
transgenic
dynamic light scattering
NMR
ELISA
fluorescence imaging
light microscopy
confocal microscopy
bioluminescence imaging

Software Mentioned

GraphPad
Living Image
ImageJ
GraphPad Prism

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