A truncated fragment of Ov-ASP-1 consisting of the core pathogenesis-related-1 (PR-1) domain maintains adjuvanticity as the full-length protein

Vaccine
Jingjing GuoYusen Zhou

Abstract

The Onchocerca volvulus activation-associated secreted protein-1 (Ov-ASP-1) has good adjuvanticity for a variety of antigens and vaccines, probably due to its ability activate antigen-processing cells (APCs). However, the functional domain of Ov-ASP-1 as an adjuvant is not clearly defined. Based on the structural prediction of this protein family, we constructed a 16-kDa recombinant protein of Ov-ASP-1 that contains only the core pathogenesis-related-1 (PR-1) domain (residues 10-153), designated ASPPR. We found that ASPPR exhibits adjuvanticity similar to that of the full-length Ov-ASP-1 (residues 10-220) for various antigens, including ovalbumin (OVA), HBsAg protein antigen, and the HIV peptide 5 (Pep5) antigen, but it is more suitable for vaccine design in ASPPR-antigen fusion proteins, and more stable in PBS than Ov-ASP-1 stored at -70 °C. These results suggest that ASPPR might be the functional region of Ov-ASP-1 as an adjuvant, and therefore could be developed as an adjuvant for human use.

References

Nov 23, 2005·Bioinformatics·Konstantin ArnoldTorsten Schwede
Jul 19, 2011·Nature Medicine·Thomas MarichalChristophe J Desmet
Mar 20, 2012·Cell·Stuart M Levitz, Douglas T Golenbock
Apr 24, 2012·Current Opinion in Immunology·Carl R AlvingSteven G Reed
Oct 26, 2012·Human Vaccines & Immunotherapeutics·Philippe Moingeon
Jul 28, 2013·Expert Review of Vaccines·Christopher B Fox, Jean Haensler
Jul 28, 2013·Expert Review of Vaccines·Gregory C Ireton, Steven G Reed
Dec 7, 2013·Nature Medicine·Steven G ReedChristopher B Fox
Dec 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·Maria VonoAnja Seubert

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Methods Mentioned

BETA
gradient dialysis
flow cytometry
ELISA
gradient
Assay

Software Mentioned

MODEL Workspace
GraphPad
SWISS
FlowJo
GraphPad Prism

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