A tryptophan-rich motif in the carboxyl terminus of the small envelope protein of hepatitis B virus is central to the assembly of hepatitis delta virus particles.

Journal of Virology
Isabelle Komla-Soukha, C Sureau

Abstract

The small hepatitis B virus surface antigen (S-HBsAg) is capable of driving the assembly and secretion of hepatitis delta virus (HDV) particles by interacting with the HDV ribonucleoprotein (RNP). Previously, a specific domain of the S-HBsAg protein carboxyl terminus, including a tryptophan residue at position 196 (W196), was proven essential for HDV maturation (S. Jenna and C. Sureau, J. Virol. 73: 3351-3358, 1999). Mutation of W196 to phenylalanine (W196F) was permissive for HBV subviral particle (SVP) secretion but deleterious to HDV virion assembly. Here, the W196F S-HBsAg deficiency was assigned to a loss of its ability for interaction with the large HDV antigen (L-HDAg), a major component of the RNP. Because the overall S-HBsAg carboxyl terminus is particularly rich in tryptophan, an amino acid frequently involved in protein-protein interactions, site-directed mutagenesis was conducted to investigate the function of the S-HBsAg Trp-rich domain in HDV assembly. Single substitutions of tryptophan between positions 163 and 201 with alanine or phenylalanine were tolerated for SVP secretion, but those affecting W196, W199, and W201 were detrimental for HDV assembly. This was proven to result from a reduced capacity of the muta...Continue Reading

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Citations

Aug 20, 2010·Journal of Virology·Ariel L ArndtBrenda G Hogue
Mar 23, 2007·Journal of Virology·Cheng HuangMing-Fu Chang
Feb 2, 2011·PloS One·Elodie TeissierEve-Isabelle Pécheur
Sep 11, 2013·Nature Reviews. Gastroenterology & Hepatology·Alessia Ciancio, Mario Rizzetto
Apr 29, 2014·Gastroenterology·Stephan UrbanFabien Zoulim
Oct 4, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E Shirvani-DastgerdiF Tacke
Jan 27, 2015·Journal of Biomolecular Structure & Dynamics·You ZhouXiang-Yin Kong
Apr 17, 2016·Journal of Hepatology·Camille Sureau, Francesco Negro
Mar 17, 2009·Journal of Hepatology·Mario Rizzetto
Feb 9, 2012·Journal of Hepatology·Gaëtan BillioudFabien Zoulim
Aug 19, 2016·Nature Reviews. Gastroenterology & Hepatology·Florian A LemppStephan Urban
Mar 16, 2018·Journal of Virology·Sascha SuffnerVolker Bruss
Jun 12, 2012·World Journal of Virology·Elham Shirvani DastgerdiFrank Tacke
May 10, 2019·Nature Communications·Jimena Perez-VargasFrançois-Loïc Cosset
Jan 4, 2020·Molecular Biology and Evolution·Joshua S MacCreadyAnthony G Vecchiarelli
Nov 4, 2016·The Journal of Biological Chemistry·Hsiu-Chen HuangCheng Huang
Nov 22, 2019·Journal of Virology·Marwa KhabirPatrick Labonté
Dec 9, 2020·Journal of Medical Virology·Mehwish SajjadSyed Hani Abidi
Feb 28, 2021·Molecular Microbiology·Joshua S MacCreadyAnthony G Vecchiarelli
May 13, 2021·MBio·Joshua S MacCready, Anthony G Vecchiarelli
Jul 3, 2021·Viruses·Jimena Pérez-VargasNatalia Freitas
Jul 9, 2021·Frontiers in Microbiology·Hans J NetterLilly K W Yuen

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