A tubular segmented-flow bioreactor for the infection of insect cells with recombinant baculovirus.

Cytotechnology
Y C HuW E Bentley

Abstract

A continuous process of insect cell (S f9) growth and baculovirus infection is tested with the sequential combination of a CSTR and a tubular reactor. A tubular infection reactor enables continuous introduction of baculovirus and therefore avoids the 'passage effect' observed in two-stage CSTR systems. Moreover, a tubular reactor can be used to test cell infection kinetics and the subsequent metabolism of infected insect cells. Unlike batch and CSTR culture, cells in a horizontally positioned tubular reactor settle due to poor mixing. We have overcome this problem by alternately introducing air bubbles and media and by maintaining a linear velocity sufficient to keep cells suspended. This article addresses the development of the tubular reactor and demonstrates its use as an infection system that complements the two-stage CSTR.

References

Apr 1, 1990·Applied Microbiology and Biotechnology·F L van LierJ Tramper
Jan 1, 1990·Annals of the New York Academy of Sciences·M L ShulerH A Wood
May 1, 1994·Applied Microbiology and Biotechnology·M Y Wang, W E Bentley
Aug 5, 1992·Biotechnology and Bioengineering·C D De GooijerJ Tramper
Sep 1, 1991·Biotechnology and Bioengineering·A A KamenJ Archambault
Sep 5, 1994·Biotechnology and Bioengineering·J F PowerL K Nielsen

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