A tumor-derived population (SCCOHT-1) as cellular model for a small cell ovarian carcinoma of the hypercalcemic type

International Journal of Oncology
Anna OtteRalf Hass

Abstract

The small cell ovarian carcinoma of the hypercalcemic type (SCCOHT) represents an aggressive tumor with poor prognosis predominantly affecting young women and so far, no cell line or animal model is available to investigate this devastating disease. Biopsy material from a recurrent SCCOHT was subjected to an explant culture to obtain an adherent and continuously proliferating cell population. Morphological and functional characterization revealed a heterogeneous population (SCCOHT-1) of about 13 µm in diameter and approximately 36 h of doubling time. Flow cytometric analysis of surface markers demonstrated the expression of CD15, CD29, CD44 and CD90 paralleled by the presence of cytokeratins and vimentin. Cytogenetic analysis and high-resolution oligo-array comparative genomic hybridization (aCGH) demonstrated a stable karyotype including deletions of the PARK2, CSMD1, GRIN2B and ATF7IP genes. Following lentiviral transduction with a GFP vector, the labeled SCCOHT-derived cells were subjected to CCE to separate distinct subpopulations as evidenced by cell cycle analysis. Subcutaneous injection of these subpopulations into NOD/SCID mice exhibited hypercalcemia and a tumor development in 100% of the mice. Re-cultivation of the mo...Continue Reading

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Citations

Apr 9, 2013·Tissue Engineering. Part C, Methods·Anna OtteRalf Hass
Jun 28, 2014·International Journal of Oncology·Anna OtteRalf Hass
Jan 25, 2020·Annual Review of Pathology·Yemin WangDavid G Huntsman
May 13, 2015·International Journal of Oncology·Yuanyuan YangRalf Hass
Oct 24, 2017·Veterinary Sciences·Nicole A KohartThomas J Rosol
May 16, 2020·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jennifer X JiDavid G Huntsman
Dec 18, 2020·Gynecologic Oncology·Anthony N KarnezisYemin Wang

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