A Tunable, Three-Dimensional In Vitro Culture Model of Growth Plate Cartilage Using Alginate Hydrogel Scaffolds

Tissue Engineering. Part a
Alek G EricksonAndrew T Dudley

Abstract

Defining the final size and geometry of engineered tissues through precise control of the scalar and vector components of tissue growth is a necessary benchmark for regenerative medicine, but it has proved to be a significant challenge for tissue engineers. The growth plate cartilage that promotes elongation of the long bones is a good model system for studying morphogenetic mechanisms because cartilage is composed of a single cell type, the chondrocyte; chondrocytes are readily maintained in culture; and growth trajectory is predominately in a single vector. In this cartilage, growth is generated via a differentiation program that is spatially and temporally regulated by an interconnected network composed of long- and short-range signaling mechanisms that together result in the formation of functionally distinct cellular zones. To facilitate investigation of the mechanisms underlying anisotropic growth, we developed an in vitro model of the growth plate cartilage by using neonatal mouse growth plate chondrocytes encapsulated in alginate hydrogel beads. In bead cultures, encapsulated chondrocytes showed high viability, cartilage matrix deposition, low levels of chondrocyte hypertrophy, and a progressive increase in cell prolife...Continue Reading

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Citations

Jun 22, 2021·Frontiers in Bioengineering and Biotechnology·Xianggang WangJincheng Wang

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Methods Mentioned

BETA
Fluorescence-activated cell sorting
FACS
flow cytometry
fluorescence microscopy
PMA

Software Mentioned

Image J
Prism
GraphPad
GraphPad Prism
ImageJ
FIJI

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