Abstract
We evaluated the RAPIDEC(®) CARBA NP assay (bioMérieux SA, Marcy-l'Étoile, France), a colorimetric test for rapid detection of carbapenemases, at two sites: Karolinska University Laboratory and PHE's national reference laboratory. A total of 138 bacterial isolates previously characterized as positive for class A, B and/or D carbapenemase genes and 138 supposed non-carbapenemase producers were tested with RAPIDEC(®) CARBA NP according to the manufacturer's protocol. Two carbapenemase-producing isolates carried both NDM and OXA-48-like genes. Molecular detection of the expected carbapenemase gene(s) was used as the gold standard, and was performed by conventional and real-time PCR in-house assays. The RAPIDEC(®) CARBA NP assay detected 135 of 138 carbapenemase producers; one OXA-48-producing Klebsiella pneumoniae and two Acinetobacter baumannii producing OXA-23 or OXA-24 were not detected. Among 'negative' controls, 135 of 138 isolates were negative by RAPIDEC(®) CARBA NP. The exceptions were one Klebsiella oxytoca, which was later found to produce GES-5 carbapenemase, one Pseudomonas aeruginosa with OprD loss and increased efflux, and one Enterobacter cloacae with impermeability. When numbers were adjusted for the GES-5 producer...Continue Reading
References
Sep 1, 1996·Antimicrobial Agents and Chemotherapy·B A RasmussenA A Medeiros
Feb 19, 2000·Antimicrobial Agents and Chemotherapy·L PoirelP Nordmann
Oct 19, 2000·Antimicrobial Agents and Chemotherapy·A M QueenanK Bush
Mar 21, 2001·Antimicrobial Agents and Chemotherapy·H YigitF C Tenover
Dec 25, 2003·Antimicrobial Agents and Chemotherapy·Laurent PoirelPatrice Nordmann
Mar 28, 2006·International Journal of Antimicrobial Agents·Neil WoodfordDavid M Livermore
Dec 23, 2006·The Journal of Antimicrobial Chemotherapy·Matthew J EllingtonNeil Woodford
Jan 21, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·V MiriagouR Cantón
Jun 15, 2011·The Journal of Antimicrobial Chemotherapy·S MushtaqN Woodford
Apr 18, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·P NordmannUNKNOWN European Network on Carbapenemases
Aug 14, 2012·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Frank HansenOrjan Samuelsen
Jul 3, 2013·Antimicrobial Agents and Chemotherapy·Nathalie TijetRoberto G Melano
Oct 2, 2013·The Journal of Antimicrobial Chemotherapy·Neil WoodfordKatie L Hopkins
Jan 28, 2014·PloS One·Tanja PasanenJuha Kirveskari
Jan 29, 2014·Antimicrobial Agents and Chemotherapy·Laurent DortetPatrice Nordmann
Oct 31, 2014·Journal of Clinical Microbiology·M DoumithN Woodford
Feb 6, 2015·Journal of Clinical Microbiology·Athanassios TsakrisYouri Glupczynski
Mar 24, 2015·PloS One·Kim van der ZwaluwLeo M Schouls
Jun 19, 2015·Journal of Clinical Microbiology·Laurent Poirel, Patrice Nordmann
Oct 2, 2015·Journal of Clinical Microbiology·Michael HombachGuido V Bloemberg
Citations
Apr 28, 2016·The Journal of Antimicrobial Chemotherapy·Danièle MeunierKatie L Hopkins
Jul 6, 2016·Frontiers in Microbiology·Chang-Ro LeeSang Hee Lee
Feb 1, 2017·Expert Review of Molecular Diagnostics·Jean-Winoc DecousserPatrice Nordmann
Jan 25, 2017·The Journal of Antimicrobial Chemotherapy·Mandy WoottonRobin A Howe
Jan 31, 2017·Journal of Microbiological Methods·Vladimira HinićAdrian Egli
Nov 18, 2016·Antimicrobial Agents and Chemotherapy·Delphine GirlichThierry Naas
Jun 14, 2017·Antimicrobial Agents and Chemotherapy·Laurent DortetThierry Naas
Dec 9, 2016·Journal of Clinical Microbiology·Audrey NoëlYouri Glupczynski
Jan 27, 2017·Journal of Clinical Microbiology·Laurent Dortet, Thierry Naas
Oct 6, 2017·Journal of Clinical Microbiology·Gina ThomsonKenneth Thomson
Nov 30, 2018·Clinical Microbiology Reviews·Marina Oviaño, Germán Bou
Feb 1, 2019·Clinical Microbiology Reviews·Wenjing WuZhiyong Zong
Nov 15, 2019·Clinical Microbiology Reviews·Johann D D PitoutYasufumi Matsumura
Feb 6, 2017·PloS One·Lauraine GauthierThierry Naas
Mar 24, 2017·The Journal of Antimicrobial Chemotherapy·Sandrine BernabeuThierry Naas
Apr 4, 2017·The Journal of Antimicrobial Chemotherapy·Youri GlupczynskiThierry Naas
Jan 20, 2018·The Journal of Antimicrobial Chemotherapy·Madalina-Maria MunteanThierry Naas
Sep 7, 2018·The Journal of Antimicrobial Chemotherapy·Danièle MeunierKatie L Hopkins
Sep 1, 2017·Scientific Reports·Pierre BogaertsYouri Glupczynski
Nov 21, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Matthew J EllingtonAlison Holmes
Aug 20, 2019·Indian Journal of Medical Microbiology·Balaji VeeraraghavanBalasubramanian Sundaram
Jan 1, 2019·Indian Journal of Medical Microbiology·Balaji VeeraraghavanBalasubramanian Sundaram
Sep 10, 2019·ACS Applied Materials & Interfaces·Lee Erik Korshoj, Prashant Nagpal