A two-centre evaluation of RAPIDEC® CARBA NP for carbapenemase detection in Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter spp

The Journal of Antimicrobial Chemotherapy
Muhammad Humaun KabirNeil Woodford

Abstract

We evaluated the RAPIDEC(®) CARBA NP assay (bioMérieux SA, Marcy-l'Étoile, France), a colorimetric test for rapid detection of carbapenemases, at two sites: Karolinska University Laboratory and PHE's national reference laboratory. A total of 138 bacterial isolates previously characterized as positive for class A, B and/or D carbapenemase genes and 138 supposed non-carbapenemase producers were tested with RAPIDEC(®) CARBA NP according to the manufacturer's protocol. Two carbapenemase-producing isolates carried both NDM and OXA-48-like genes. Molecular detection of the expected carbapenemase gene(s) was used as the gold standard, and was performed by conventional and real-time PCR in-house assays. The RAPIDEC(®) CARBA NP assay detected 135 of 138 carbapenemase producers; one OXA-48-producing Klebsiella pneumoniae and two Acinetobacter baumannii producing OXA-23 or OXA-24 were not detected. Among 'negative' controls, 135 of 138 isolates were negative by RAPIDEC(®) CARBA NP. The exceptions were one Klebsiella oxytoca, which was later found to produce GES-5 carbapenemase, one Pseudomonas aeruginosa with OprD loss and increased efflux, and one Enterobacter cloacae with impermeability. When numbers were adjusted for the GES-5 producer...Continue Reading

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Citations

Apr 28, 2016·The Journal of Antimicrobial Chemotherapy·Danièle MeunierKatie L Hopkins
Jan 25, 2017·The Journal of Antimicrobial Chemotherapy·Mandy WoottonRobin A Howe
Nov 18, 2016·Antimicrobial Agents and Chemotherapy·Delphine GirlichThierry Naas
Jun 14, 2017·Antimicrobial Agents and Chemotherapy·Laurent DortetThierry Naas
Dec 9, 2016·Journal of Clinical Microbiology·Audrey NoëlYouri Glupczynski
Jan 27, 2017·Journal of Clinical Microbiology·Laurent Dortet, Thierry Naas
Feb 1, 2019·Clinical Microbiology Reviews·Wenjing WuZhiyong Zong
Nov 15, 2019·Clinical Microbiology Reviews·Johann D D PitoutYasufumi Matsumura
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Sep 10, 2019·ACS Applied Materials & Interfaces·Lee Erik Korshoj, Prashant Nagpal

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